Madsen Svend Roesen, Kunert Grit, Reichelt Michael, Gershenzon Jonathan, Halkier Barbara Ann
DynaMo Center of Excellence, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 40 Thorvaldsensvej, DK-1871, Frederiksberg C, Denmark.
Max Planck Institute for Chemical Ecology, 07745, Jena, Germany.
J Chem Ecol. 2015 Nov;41(11):975-84. doi: 10.1007/s10886-015-0641-3. Epub 2015 Oct 28.
As aphids are a pest on various crops worldwide, a better understanding of the interaction between aphids and plant host defenses is required. The green peach aphid (Myzus persicae) feeds on a variety of plant species, including the model plant Arabidopsis thaliana (Arabidopsis), in which glucosinolates function as a major part of the chemical defense. Several studies have shown that glucosinolates play a role in interactions between Arabidopsis and the green peach aphid. In this work, we used a recently identified Arabidopsis glucosinolate transporter mutant (gtr1gtr2 dKO), with altered glucosinolate content in the vasculature, to investigate the role of defense compound transport in aphid infestation. By monitoring aphid performance on caged leaves and analyzing glucosinolates in leaf tissue and phloem sap, as well as inside aphids, we examined if a change in spatial distribution of glucosinolates within a leaf influences aphid performance. Based on reduced glucosinolate content in the phloem sap of the transporter mutant, we hypothesized that aphids would perform better on gtr1gtr2 dKO leaves compared to WT. Unexpectedly, aphids performed poorly on gtr1gtr2 dKO leaves. Our data suggest that higher glucosinolate content in tissues surrounding the phloem of the double transporter mutant may play a role in reducing aphid performance on this genotype.
由于蚜虫是全球各种作物的害虫，因此需要更好地了解蚜虫与植物宿主防御之间的相互作用。桃蚜（Myzus persicae）以多种植物为食，包括模式植物拟南芥（Arabidopsis thaliana），其中芥子油苷是化学防御的主要组成部分。多项研究表明，芥子油苷在拟南芥与桃蚜的相互作用中发挥作用。在这项研究中，我们使用了最近鉴定出的一种拟南芥芥子油苷转运体突变体（gtr1gtr2 dKO），其维管系统中的芥子油苷含量发生了改变，以研究防御化合物运输在蚜虫侵染中的作用。通过监测笼养叶片上蚜虫的表现，并分析叶片组织、韧皮部汁液以及蚜虫体内的芥子油苷，我们研究了叶片内芥子油苷空间分布的变化是否会影响蚜虫的表现。基于转运体突变体韧皮部汁液中芥子油苷含量的降低，我们推测与野生型相比，蚜虫在gtr1gtr2 dKO叶片上的表现会更好。出乎意料的是，蚜虫在gtr1gtr2 dKO叶片上的表现很差。我们的数据表明，双转运体突变体韧皮部周围组织中较高的芥子油苷含量可能在降低该基因型上蚜虫的表现中发挥作用。
