Ishizaka T, Saito H, Hatake K, Dvorak A M, Leiferman K M, Arai N, Ishizaka K
Johns Hopkins University School of Medicine, Baltimore, Md.
Int Arch Allergy Appl Immunol. 1989;88(1-2):46-9. doi: 10.1159/000234746.
The effects of recombinant human interleukins (IL) on hematopoiesis were explored by using suspension cultures of mononuclear cells of human umbilical cord blood and bone marrow cells. The results showed that IL-5 induced the selective differentiation and proliferation of eosinophils. After 3 weeks in culture with IL-5, over 90% of nonadherent cells in both bone marrow cell and cord blood cell cultures became eosinophilic myelocytes. Culture of the same cells with IL-4 resulted in the selective growth of OKT-3+ lymphocytes. In suspension cultures of bone marrow cells and cord blood cells grown in the presence of IL-3, basophilic, eosinophilic, and neutrophilic myelocytes developed within 2 weeks. By 3 weeks, however, the majority of non-adherent cells became eosinophilic myelocytes. In contrast to mouse bone marrow cell cultures, neither IL-3 nor combination of IL-3 and IL-4 induced the differentiation of mast cells in human bone marrow or cord blood cell cultures.
通过使用人脐带血单个核细胞和骨髓细胞的悬浮培养，探讨了重组人白细胞介素（IL）对造血作用的影响。结果显示，IL-5诱导嗜酸性粒细胞的选择性分化和增殖。在用IL-5培养3周后，骨髓细胞和脐带血细胞培养物中超过90%的非贴壁细胞成为嗜酸性晚幼粒细胞。用IL-4培养相同细胞导致OKT-3+淋巴细胞的选择性生长。在IL-3存在下生长的骨髓细胞和脐带血细胞的悬浮培养物中，嗜碱性、嗜酸性和嗜中性晚幼粒细胞在2周内形成。然而，到3周时，大多数非贴壁细胞成为嗜酸性晚幼粒细胞。与小鼠骨髓细胞培养不同，IL-3或IL-3与IL-4的组合均未诱导人骨髓或脐带血细胞培养物中肥大细胞的分化。
