Guo Qingfa, Kuang Lei, Cao Hui, Li Weizhong, Wei Jing
Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong Province, Weifang University, Weifang, Shandong 261061, PR China.
Department of Pharmacy, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi 330006,PR China.
Colloids Surf B Biointerfaces. 2015 Dec 1;136:687-93. doi: 10.1016/j.colsurfb.2015.10.013. Epub 2015 Oct 22.
In this paper, a novel bifunctional nanoprobe based on polyethylene glycol(MPEG)-poly(ϵ-caprolactone)(ϵ-CL)-polyethylenimine(PEI) labeled with FITC (MPEG-PCL-PEI-FITC, PCIF) were prepared to provide tumor therapy and simultaneous diagnostic information via magnetic resonance imaging (MRI) and optical imaging. Superparamagnetic iron oxide (SPIO) and doxorubicin (DOX) loaded PCIF (PCIF/SPIO/DOX) nanoprobes were prepared by self-assembling into micelles, which had uniformly distributed particle size of 130 ± 5 nm and a zeta potential of +35 ± 2 mV. Transmission electronic microscopy(TEM) showed that SPIO NPs were loaded into PCIF micelles. The PCIF/SPIO/DOX nanoprobes were superparamagnetic at 300 K with saturated magnetization of 20.5 emu/g Fe by vibrating-sample-magnetomete (VSM). Studies on cellular uptake of PCIF/SPIO/DOX nanoprobes demonstrated that SPIO NPs, DOX and FITC labeled MPEG-PCL-PEI were simultaneously taken up by the breast cancer (4T1) cells. After intravenous injection of PCIF/SPIO/DOX nanoprobes in 4T1 tumor-bearing mice, SPIO NPs, DOX and FITC labeled MPEG-PCL-PEI micelles were simultaneously delivered into tumor tissue by histochemisty. This work is important for the applications to multimodal diagnostic and theragnosis as nanomedicine.
在本文中，制备了一种基于聚乙二醇（MPEG）-聚（ε-己内酯）（ε-CL）-聚乙烯亚胺（PEI）并标记有FITC的新型双功能纳米探针（MPEG-PCL-PEI-FITC，PCIF），以通过磁共振成像（MRI）和光学成像提供肿瘤治疗及同步诊断信息。通过自组装成胶束制备了负载超顺磁性氧化铁（SPIO）和阿霉素（DOX）的PCIF（PCIF/SPIO/DOX）纳米探针，其粒径均匀分布为130±5nm，ζ电位为+35±2mV。透射电子显微镜（TEM）显示SPIO纳米颗粒被负载到PCIF胶束中。通过振动样品磁强计（VSM）测得，PCIF/SPIO/DOX纳米探针在300K时具有超顺磁性，饱和磁化强度为20.5emu/g Fe。对PCIF/SPIO/DOX纳米探针细胞摄取的研究表明，SPIO纳米颗粒、DOX和FITC标记的MPEG-PCL-PEI被乳腺癌（4T1）细胞同时摄取。在4T1荷瘤小鼠静脉注射PCIF/SPIO/DOX纳米探针后，通过组织化学方法将SPIO纳米颗粒、DOX和FITC标记的MPEG-PCL-PEI胶束同时递送至肿瘤组织。这项工作对于纳米医学在多模态诊断与治疗中的应用具有重要意义。
