Farooq Muhammad, Taha Nael Abu, Butorac Rachel R, Evans Daniel A, Elzatahry Ahmed A, Wadaan Mohammad A M, Cowley Alan H
Bioproducts Research Chair, Department of Zoology, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia E-mail :
Asian Pac J Cancer Prev. 2015;16(16):7003-6. doi: 10.7314/apjcp.2015.16.16.7003.
Nanoparticles of gold and silver are offering revolutionary changes in the field of cancer therapy. N-heterocyclic carbene (NHC) metal complexes possess diverse biological activities and are being investigated as potential chemotherapeutic agents. The purpose of this study was to examine the cytotoxicity and possible mechanisms of action of two types of newly synthesized nanofiber composites containing BIAN N-heterocyclic gold carbene complexes in two types of human cancer cells, namely breast cancer (MCF7) and liver cancer (HepG2) cells and also in normal human embryonic kidney cells (HEK 293).
Cytotoxicity was assessed by MTT cell viability assay and oxidative stress by checking the total glutathione level.
Both compounds affected the cell survival of the tested cell lines at very low concentrations (IC50 values in the micro molar range) as compared to a well-known anti-cancer drug, 5 fluorouracil. A 60-80% depletion in total glutathione level was detected in treated cells.
Reduction in total glutathione level is one of the biochemical pathways for the induction of oxidative stress which in turn could be a possible mechanism of action by which these compounds induce cytotoxicity in cancer cell lines. The in vitro toxicity towards cancer cells found here means that these molecules could be potential anticancer candidates.
金和银纳米颗粒正在癌症治疗领域带来革命性变化。N-杂环卡宾（NHC）金属配合物具有多种生物活性，正作为潜在的化疗药物进行研究。本研究的目的是检测两种新合成的含BIAN N-杂环金卡宾配合物的纳米纤维复合材料对两种人类癌细胞系（即乳腺癌（MCF7）和肝癌（HepG2）细胞）以及正常人胚胎肾细胞（HEK 293）的细胞毒性和可能的作用机制。
通过MTT细胞活力测定评估细胞毒性，并通过检测总谷胱甘肽水平评估氧化应激。
与一种知名抗癌药物5-氟尿嘧啶相比
