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培养基中添加复合氨基酸可提高龙葵对镉-多环芳烃污染土壤的植物修复能力。

Compound amino acids added in media improved Solanum nigrum L. phytoremediating CD-PAHS contaminated soil.

作者信息

Wei Shuhe, Bai Jiayi, Yang Chuanjie, Zhang Qianru, Knorrm Klaus-Holger, Zhan Jie, Gao Qianhui

机构信息

a Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences , Shenyang , P.R. China.

b University of Chinese Academy of Sciences , Beijing , P.R. China.

出版信息

Int J Phytoremediation. 2016;18(4):358-63. doi: 10.1080/15226514.2015.1109592.

Abstract

Cd hyperaccumulator Solanum nigrum L. was a promising plant used to simultaneously remediate Cd-PAHs combined pollution soil through its extra accumulation capacity and rhizosphere degradation. This article compared the strengthening remediation role of cysteine (Cys), glycine (Gly) and glutamic acid (Glu) with EDTA and TW80. The results showed that the addition of 0.03 mmol L(-1) Cys, Gly, and Glu didn't significantly impact (p < 0.05) shoot biomass of S. nigrum, but obviously increased Cd concentration. Therefore, Cd capacity (µg pot(-1)) in shoots of S. nigrum was significantly increased (p < 0.05) by 37.7% compared to the control without reagent added. At the meantime, the PAHs degradation ratio in rhizoshpere was increased by 34.5%. Basically, the improving role of Cys, Gly, and Glu was higher than EDTA and TW80. The main reasons of enhanced the accumulation of S. nigrum to Cd might lie in the addition of Cys, Gly, and Glu which reduced pH and increased extractable Cd concentration in rhizosphere and phytochelatines (PCs) concentration in leaves. As for the degradation of PAHs in rhizosphere, increased microorganism number might be play important role.

摘要

镉超富集植物龙葵是一种很有前景的植物,可通过其超积累能力和根际降解作用同时修复镉-多环芳烃复合污染土壤。本文比较了半胱氨酸(Cys)、甘氨酸(Gly)和谷氨酸(Glu)与乙二胺四乙酸(EDTA)和吐温80的强化修复作用。结果表明,添加0.03 mmol L(-1)的Cys、Gly和Glu对龙葵地上部生物量没有显著影响(p < 0.05),但明显提高了镉浓度。因此,与不添加试剂的对照相比,龙葵地上部的镉含量(µg 盆(-1))显著增加(p < 0.05),增幅为37.7%。同时,根际多环芳烃降解率提高了34.5%。总体而言,Cys、Gly和Glu的改善作用高于EDTA和吐温80。龙葵对镉积累增强的主要原因可能在于添加Cys、Gly和Glu降低了根际pH值,增加了根际可提取镉浓度和叶片中植物螯合肽(PCs)的浓度。至于根际多环芳烃的降解,微生物数量增加可能起了重要作用。

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