Department of Biochemistry and Centre of Excellence in Biomedical Engineering and Systems Biology, University of Calcutta 35 Ballygunge Circular Road, Kolkata, 700019, India.
Pharmacol Res Perspect. 2015 Oct;3(5):e00188. doi: 10.1002/prp2.188. Epub 2015 Oct 2.
Microparticles (MPs) have great potentiality in material science- based applications. Their use in biology is however limited to clinics and has rarely been exploited in the pharmaceutical context. Unlike nanoparticles (NPs), they are amenable to routine detection by flow cytometry and confocal microscopy. Though MPs can constitute a wide variety of materials, including ceramics, glass, polymers, and metals and can be synthesized by chemical process but wet processes for the preparation of microparticles have rarely been attemped. In this paper, a thrombotic route is shown to successfully generate biocompatible MP of a model anticancer drug (doxorubicin hydrochloride). Synthesis of MPs from platelets and drug loading in to these MPs was confirmed by flow cytometry and confocal microscopy. Human cervical cancer cell line (HeLa) was treated with these drug-loaded MPs to investigate whether the loaded MPs have the capacity to deliver drug to the cancer cells. In addition, Magnetic force microscopy was used to detect the preparation of MPs loaded with magnetic NPs. The efficiency of the drug-loaded MPs in inducing cytotoxicity in cancer cell line, shown to be significantly higher than the free drug itself. The drug-loaded MP is shown to have a much higher cytotoxic propensity than the free drug applied at comparable doses. The thrombotic approach can also be applied to synthesize MP containing NPs which in turn can lead to generate a wide variety of new biocompatible materials.
微粒（MPs）在基于材料科学的应用中具有巨大的潜力。然而，它们在生物学中的应用仅限于临床，很少在药物学领域得到利用。与纳米颗粒（NPs）不同，它们可以通过流式细胞术和共聚焦显微镜进行常规检测。虽然 MPs 可以由各种材料组成，包括陶瓷、玻璃、聚合物和金属，并且可以通过化学过程合成，但很少有尝试过用于制备 MPs 的湿法工艺。在本文中，血栓形成途径被证明可以成功地生成模型抗癌药物（盐酸多柔比星）的生物相容性 MPs。通过流式细胞术和共聚焦显微镜证实了从血小板合成 MPs 和将药物加载到这些 MPs 中。用人宫颈癌细胞系（HeLa）对这些载药 MPs 进行处理，以研究载药 MPs 是否有能力将药物递送到癌细胞中。此外，还使用磁力显微镜来检测载有磁性 NPs 的 MPs 的制备。载药 MPs 在诱导癌细胞毒性方面的效率明显高于游离药物本身。与应用可比剂量的游离药物相比，载药 MPs 的细胞毒性倾向要高得多。血栓形成方法也可以应用于合成含有 NPs 的 MPs，这反过来又可以生成各种新的生物相容性材料。
