巴西中西部戈亚尼亚市埃及伊蚊种群中拟除虫菊酯抗性的表型和基因型特征
Phenotypic and genotypic profile of pyrethroid resistance in populations of the mosquito Aedes aegypti from Goiânia, Central West Brazil.
作者信息
Chapadense Francesca Guaracyaba Garcia, Fernandes Everton Kort Kamp, Lima José Bento Pereira, Martins Ademir Jesus, Silva Luana Carrara, Rocha Welington Tristão da, Santos Adelair Helena Dos, Cravo Pedro
机构信息
Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
Laboratório de Fisiologia e Controle de Artrópodes Vetores, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
出版信息
Rev Soc Bras Med Trop. 2015 Sep-Oct;48(5):607-9. doi: 10.1590/0037-8682-0046-2015.
INTRODUCTION
The mosquito Aedes aegypti has evolved resistance to pyrethroid insecticides. The present study evaluated Ae. aegypti from Goiânia for the resistant phenotype and for mutations associated with resistance.
METHODS
Insecticide dose-response bioassays were conducted on mosquitoes descended from field-collected eggs, and polymerase chain reaction (PCR) was used to genotype 90 individuals at sites implicated in pyrethroid resistance.
RESULTS
All mosquito populations displayed high levels of resistance to deltamethrin, as well as high frequencies of the 1016Ile kdr and 1534Cys kdrmutations.
CONCLUSIONS
Aedes aegypti populations in the Goiânia area are highly resistant to deltamethrin, presumably due to high frequencies of kdr(knockdown-resistance) mutations.
引言
埃及伊蚊已对拟除虫菊酯类杀虫剂产生抗性。本研究评估了戈亚尼亚的埃及伊蚊的抗性表型以及与抗性相关的突变。
方法
对从野外采集的卵孵化出的蚊子进行杀虫剂剂量反应生物测定，并使用聚合酶链反应（PCR）对90只个体在与拟除虫菊酯抗性相关的位点进行基因分型。
结果
所有蚊子种群对溴氰菊酯均表现出高水平抗性，同时1016Ile kdr和1534Cys kdr突变的频率也很高。
结论
戈亚尼亚地区的埃及伊蚊种群对溴氰菊酯具有高度抗性，可能是由于击倒抗性（kdr）突变的高频率所致。
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