Emami Amir, Yaghobi Ramin, Moattari Afagh, Baseri Salehi Majid, Roozbeh Jamshid
>From the Department of Microbiology, Science and Research Branch, Islamic Azad University, Fars, Iran.
Exp Clin Transplant. 2017 Apr;15(2):150-156. doi: 10.6002/ect.2014.0230. Epub 2015 Oct 30.
Adaptation of BK polyomavirus with infected host cells may cause rearrangement of the noncoding control region of viral genomic DNA. Archetype, the prearranged transmissible form of the virus, actively replicates in the tubular epithelial cells, whereas isolates with rearranged noncoding control region sequences are found in other parts of the kidney. Clinical observations highlighted the importance of the noncoding control region rearrangements in BK virus-associated nephropathy. Therefore, we evaluated the sequence pattern of the noncoding control region in kidney transplant patients suspected of having BK virus-associated nephropathy.
In this single-center, cross-sectional study, 129 kidney transplant patients suspected of having BK virus-associated nephropathy and who were admitted to Namazi Hospital were enrolled for analysis between years 2010 and 2013. Blood samples were collected from each patient. The BK polyomavirus infection was diagnosed using quantitative real-time polymerase chain reaction. The BK polyomavirus-infected patient plasma samples were amplified using in-house nested polymerase chain reaction and sequenced. The contiguous alignment noncoding control region sequences were analyzed with software.
The BK polyomavirus infection was observed in plasma samples of 11 of 129 (8.5%) patients after kidney transplant. Sequence alignments showed that BK polyomavirus noncoding control region sequences in all viral infected patients with BK virus-associated nephropathy showed a complete rearranged algorithm compared with the archetype sequences. The most prevalent noncoding control region sequences were registered in a genetic sequence database (National Institutes of Health). No association was observed between risk factors and BK polyomavirus infection. There were 3 BK polyomavirus-infected patients who simultaneously had active cytomegalovirus infection.
Determination of the rearranged pattern of the noncoding control region sequences in BK polyomavirus isolates from plasma samples may help improve the diagnostic and therapeutic protocols against this viral infection in patients with BK virus-associated nephropathy.
BK多瘤病毒与受感染宿主细胞的适应性变化可能导致病毒基因组DNA非编码控制区发生重排。原型病毒是该病毒预先设定的可传播形式，在肾小管上皮细胞中活跃复制，而具有重排非编码控制区序列的分离株则存在于肾脏的其他部位。临床观察突出了非编码控制区重排在BK病毒相关性肾病中的重要性。因此，我们评估了疑似患有BK病毒相关性肾病的肾移植患者非编码控制区的序列模式。
在这项单中心横断面研究中，2010年至2013年间纳入了129名疑似患有BK病毒相关性肾病并入住纳马齐医院的肾移植患者进行分析。采集每位患者的血液样本。采用定量实时聚合酶链反应诊断BK多瘤病毒感染。使用内部巢式聚合酶链反应对BK多瘤病毒感染患者的血浆样本进行扩增并测序。使用软件分析连续比对的非编码控制区序列。
129名肾移植患者中有11名（8.5%）的血浆样本中观察到BK多瘤病毒感染。序列比对显示，与原型序列相比，所有患有BK病毒相关性肾病的病毒感染患者的BK多瘤病毒非编码控制区序列均呈现完全重排的算法。最常见的非编码控制区序列已登记在基因序列数据库（美国国立卫生研究院）中。未观察到危险因素与BK多瘤病毒感染之间存在关联。有3名BK多瘤病毒感染患者同时合并有活动性巨细胞病毒感染。
确定血浆样本中BK多瘤病毒分离株非编码控制区序列的重排模式可能有助于改进针对BK病毒相关性肾病患者这种病毒感染的诊断和治疗方案。
