Amer Said, Abd El Wahab Taher, El Naby Metwaly Abd, Feng Yaoyu, Xiao Lihua
Division of Foodborne, Waterborne and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America; Department of Zoology, Faculty of Science, Kafr El sheikh University, Kafr El Sheikh, Egypt.
Animal Health Research Institute, Kafr El Sheikh Provisional Laboratory, Kafr El Sheikh, Egypt.
PLoS One. 2015 Oct 30;10(10):e0141554. doi: 10.1371/journal.pone.0141554. eCollection 2015.
Species delimitation of Psoroptes spp. and identity of the parasite in water buffaloes remain poorly defined. In this study, Psoroptes infestation on three water buffalo farms in Egypt was examined based on morphometric characteristics, especially the opisthosomal setae of adult male mites. Clinical investigations showed that 28% (196/700) of the sampled animals had mange infestation. Microscopic examinations of 80 skin scrapings indicated the occurrence of Psoroptes mites in 17 (21.3%) samples, Sarcoptes mites in 27 (33.7%) samples, and the concurrence of both in 36 (45.0%) samples. Morphologically, the Psoroptes parasite was identified as Psoroptes natalensis. DNA sequence analysis of the second internal transcribed spacer (ITS2) in 11 representative samples confirmed the diagnosis and suggested the presence of a distinct variety of Psoroptes natalensis in Egypt.
痒螨属物种的界定以及水牛体内寄生虫的身份仍不明确。在本研究中，基于形态学特征，尤其是成年雄性螨的背板刚毛，对埃及三个水牛养殖场的痒螨感染情况进行了检查。临床调查显示，28%（196/700）的采样动物患有疥螨感染。对80份皮肤刮片进行显微镜检查表明，在17份（21.3%）样本中发现了痒螨，在27份（33.7%）样本中发现了疥螨，在36份（45.0%）样本中二者同时存在。从形态学上看，痒螨寄生虫被鉴定为纳塔尔痒螨。对11份代表性样本的第二内部转录间隔区（ITS2）进行DNA序列分析，证实了诊断结果，并表明在埃及存在一种独特的纳塔尔痒螨变种。
