Dar Gowhar H, Dar Shoaib A, Kamili Azra N, Chishti Mohammad Z, Ahmad Fayaz
Department of Environmental Science, University of Kashmir, Srinagar 190006, India; Centre of Research for Development, University of Kashmir, Srinagar 190006, India.
Department of Zoology and Environmental Sciences, Punjabi University, Patialia 147002, India.
Microb Pathog. 2016 Feb;91:136-40. doi: 10.1016/j.micpath.2015.10.017. Epub 2015 Oct 27.
The current study focuses on the detection and characterization of potentially pathogenic Aeromonas sobria from fish silver carp (Hypophthalmichthys molitrix). Assessment of clinical, microbiological, pathological and biochemical characteristics of A. sobria were taken into account in order to understand the epidemiology, frequency and occurrence of this infection. Clinically the infected fish (H. molitrix) was observed for various types of symptoms. A total of 33 colonies of A. sobria strain were isolated from 20 cultured H. molitrix, collected from controlled fish pond. Microscopic examination revealed that the strains were rod-shaped, Gram negative bacteria. The revealed percent probability identification of A. sobria from the biochemical characterization in VITEK system was 93% with gram negative (GN) card. The histopathology of Gills caused by this bacterium, A. sobria indicate haemorrhagic gill epithelia and epithelial hyperplasia. Lamelar epithelial hypertrophy and hyperplasia with degenerative changes of the epithelium and hypertrophic epitheliocystis infected cells on gills of H. molitrix were observed during the present study.
当前的研究聚焦于从鲢鱼（Hypophthalmichthys molitrix）中检测和鉴定潜在致病的温和气单胞菌。为了了解这种感染的流行病学、发生频率和情况，对温和气单胞菌的临床、微生物学、病理学和生化特征进行了评估。临床上观察感染的鱼（鲢鱼）出现的各种症状。从20条养殖的鲢鱼中总共分离出33个温和气单胞菌菌株，这些鲢鱼采自受控鱼塘。显微镜检查显示这些菌株为杆状革兰氏阴性菌。在VITEK系统中通过生化特征鉴定温和气单胞菌的概率为93%（使用革兰氏阴性（GN）卡）。由这种细菌——温和气单胞菌引起的鳃组织病理学表现为鳃上皮出血和上皮增生。在本研究中观察到鲢鱼鳃上出现片状上皮肥大和增生，伴有上皮的退行性变化以及感染细胞的肥大上皮囊肿。
