通过透射电子显微镜观察芽殖酵母中的胞质分裂事件

Visualization of Cytokinesis Events in Budding Yeast by Transmission Electron Microscopy.

作者信息

Meitinger Franz, Pereira Gislene

机构信息

DKFZ-ZMBH Alliance, German Cancer Research Center (DKFZ), University of Heidelberg, Im Neuenheimer Feld 581, 69120, Heidelberg, Germany.

Ludwig Institute for Cancer Research, 9500 Gilman Drive, CMM East, La Jolla, CA, 92093, USA.

出版信息

Methods Mol Biol. 2016;1369:87-95. doi: 10.1007/978-1-4939-3145-3_7.

DOI:10.1007/978-1-4939-3145-3_7

PMID:26519307

Abstract

In yeast cells, cytokinesis is accompanied by morphological changes due to cell wall growth during furrow ingression and abscission. The characteristics of the growing cell wall can be used as an indicator for the function of the contractile actomyosin ring, the Rho-GTPases Rho1 and Cdc42 and/or other factors that drive cytokinesis. The ultrastructural information of the cell wall can be easily acquired by transmission electron microscopy, which makes this technique an invaluable tool to analyze cell division in yeast cells. Here, we describe the process of embedding and staining budding yeast cells for transmission electron microscopic analysis of cytokinetic events.

摘要

在酵母细胞中，胞质分裂伴随着形态变化，这是由于在缢缩沟形成和脱离过程中细胞壁生长所致。生长中的细胞壁的特征可作为收缩性肌动球蛋白环、Rho-GTP酶Rho1和Cdc42以及/或其他驱动胞质分裂的因子功能的指标。通过透射电子显微镜可以轻松获取细胞壁的超微结构信息，这使得该技术成为分析酵母细胞中细胞分裂的宝贵工具。在这里，我们描述了对出芽酵母细胞进行包埋和染色以用于胞质分裂事件透射电子显微镜分析的过程。