转化生长因子-β信号通路在骨重塑耦联中的作用。

Role of TGF-β Signaling in Coupling Bone Remodeling.

作者信息

Crane Janet L, Xian Lingling, Cao Xu

机构信息

Department of Orthopaedic Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

出版信息

Methods Mol Biol. 2016;1344:287-300. doi: 10.1007/978-1-4939-2966-5_18.

DOI:10.1007/978-1-4939-2966-5_18

PMID:26520132

Abstract

TGF-β signaling plays a key role in the temporal and spatial regulation of bone remodeling. During osteoclast bone resorption, TGF-β is released from the bone matrix and activated. Active TGF-β recruits mesenchymal stem cells to the bone resorption pit through the SMAD signaling pathway. Mesenchymal stem cells undergo osteoblast differentiation and deposit new bone filling in the resorption pit and maintaining the structural integrity of the skeleton. Thus, TGF-β signaling plays a key role in the coupling process and disruptions to the TGF-β signaling pathway lead to loss of skeletal integrity. This chapter describes methods on how to quantitate bone matrix TGF-β and assess its role in mesenchymal stem cell migration both in vitro and in vivo.

摘要

转化生长因子-β（TGF-β）信号通路在骨重塑的时空调节中起关键作用。在破骨细胞进行骨吸收过程中，TGF-β从骨基质中释放并被激活。活性TGF-β通过SMAD信号通路将间充质干细胞招募至骨吸收凹陷处。间充质干细胞经历成骨细胞分化，并沉积新骨以填充吸收凹陷并维持骨骼的结构完整性。因此，TGF-β信号通路在耦合过程中起关键作用，而TGF-β信号通路的破坏会导致骨骼完整性丧失。本章介绍了如何定量骨基质TGF-β以及评估其在体外和体内对间充质干细胞迁移作用的方法。