Gobbe P, Herchuelz A
Laboratory of Pharmacology, Brussels University School of Medicine, Belgium.
Res Commun Chem Pathol Pharmacol. 1989 Feb;63(2):231-47.
Cytosolic free Ca2+ concentration ([Ca2+]i) was measured in normal rat pancreatic islet cells using the intracellular Ca2+ indicator fura 2. Glucose induced an initial decrease followed by a secondary rise in [Ca2+]i. The secondary rise probably resulted from an increase in Ca2+ inflow into the cells, while the initial decrease displayed several characteristics of the well known initial decrease in 45Ca efflux induced by glucose in prelabelled pancreatic islets. It is concluded that glucose may exert both an inhibitory and a stimulatory effect on [Ca2+]i in normal isolated islet cells.
使用细胞内钙离子指示剂fura 2测量正常大鼠胰岛细胞中的胞质游离钙离子浓度（[Ca2+]i）。葡萄糖诱导[Ca2+]i先下降，随后出现二次上升。二次上升可能是由于钙离子流入细胞增加所致，而最初的下降表现出预标记胰岛中葡萄糖诱导的45Ca外流最初下降的几个特征。得出的结论是，葡萄糖可能对正常分离的胰岛细胞中的[Ca2+]i产生抑制和刺激作用。
