Wu Jiangyi, Wang Fuyou, Yang Liu, Liao Ying
Department of Orthopedics, First Affiliated Hospital, Nanhua University, Hengyang 421001, China.
Center for Joint Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2015 Nov;31(11):1502-5, 1510.
To investigate the effect of chondrocytic biomimetic matrix scaffold materials containing type 2 collagen (Col2) hydrogel, chondroitin sulphate (CS) and (or) hyaluronic acid (HA) on chondrogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) in vitro.
BMSCs were cultured in different groups as following: control, Col2, Col2-CS, Col2-HA, Col2-CS-HA. On day 7, 14, 21 and 28, cell proliferation and differentiation activities were determined using CCK-8 assay. The mRNA and protein expressions of Col2, sex-determining region Y (SRY)-related high mobility group-box gene 9 (SOX9) and aggrecan were detected using reverse transcription PCR and immunofluorescence technique.
CCK-8 assay indicated that chondrocytic biomimetic matrix scaffold materials based on Col2 could be used as good extracellular matrix for BMSCs culture in vitro. CS and HA increased the proliferation and differentiation activities. All the groups induced effectively BMSCs to differentiate into chondrocytes. Compared with the other groups, Col2-CS-HA-coated group up-regulated the mRNA and protein expressions of Col2, SOX9 and aggrecan the most effectively.
Chondrocytic biomimetic matrix scaffold materials based on Col2 could effectively induce rat BMSCs to differentiate into chondrocyte-like cells in vitro, and supplementation of the hydrogels with both CS and HA had the maximum efficacy.
