Ayatollahi Hossein, Rafiee Mohammad, Keramati Mohammad-Reza, Balali-Mood Mahdi, Asgharzadeh Ali, Sadeghian Mohammad Hadi, Sheikhi Maryam, Amini Nafiseh, Zarmehri Azam Moradi
Cancer Molecular Pathology Research Center, Ghaem Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Basic Med Sci. 2015 Sep;18(9):862-6.
Sulfur mustard (SM) was used by the Iraqi army against the Iranian troops in the Iran-Iraq war from 1983-1988. This chemical gas affects different organs including the skin, lungs and the hematopoietic system. Any exposure to SM increases the risk of chromosomal breaking, hyperdiploidy and hypodiploidy. Studies have shown that the risk for acute myeloblastic and lymphoblastic leukemia increases in veterans exposed to SM. FLT3 mutations including ITD and TKD mutations had been observed in some cases of leukemia. Therefore, we aimed to investigate the frequency of FLT3-TKD835 mutations in the veterans exposed to SM agent.
We studied 42 patients who were exposed to SM during the war in Khorasan Razavi province, Mashhad, Iran in 2012. As control group, 30 healthy males were selected from first-degree relatives of the patients. For assessment of TKD835 mutation, DNA was extracted and RFLP-PCR was performed.
Analysis of RFLP-PCR data showed no FLT-3 TKD mutation in any of the patients.
Although contact with SM can increase the risk of malignancy especially hematologic neoplasms, results of the study show that another mechanism of leukemogenesis, other than FLT3-TKD mutation, may be the reason for increased risk of leukemia in SM toxicity.
1983年至1988年的两伊战争中，伊拉克军队曾对伊朗军队使用硫芥（SM）。这种化学气体可影响包括皮肤、肺和造血系统在内的不同器官。任何硫芥暴露都会增加染色体断裂、超二倍体和亚二倍体的风险。研究表明，接触硫芥的退伍军人患急性髓性白血病和淋巴细胞白血病的风险增加。在一些白血病病例中观察到了包括内部串联重复（ITD）和酪氨酸激酶结构域（TKD）突变在内的FLT3突变。因此，我们旨在调查接触硫芥制剂的退伍军人中FLT3-TKD835突变的频率。
2012年，我们对伊朗马什哈德呼罗珊拉扎维省战争期间接触硫芥的42例患者进行了研究。作为对照组，从患者的一级亲属中选取30名健康男性。为评估TKD835突变，提取DNA并进行限制性片段长度多态性聚合酶链反应（RFLP-PCR）。
RFLP-PCR数据分析显示，所有患者均未出现FLT-3 TKD突变。
虽然接触硫芥会增加患恶性肿瘤尤其是血液肿瘤的风险，但研究结果表明，除FLT3-TKD突变外，白血病发生的另一种机制可能是硫芥中毒导致白血病风险增加的原因。
