Zhang L, Li J, Yun T T, Li A K, Qi W T, Liang X X, Wang Y W, Liu S
J Anim Sci. 2015 Oct;93(10):4796-807. doi: 10.2527/jas.2015-9243.
This study was conducted to evaluate the pilot-scale production of microencapsulated in a 500-L fermenter using emulsion and gelation and to assess the effect of the products on the growth performance, antioxidant activity, immune function, and cecal microbiota in Arbor Acres broilers. A total of seven hundred 1-d-old male Arbor Acres broilers were randomly assigned to 7 dietary treatments with 5 replicate pens per treatment and 20 broilers per pen. The dietary treatments were as follows: 1) basal diet (CON), 2) basal diet containing 0.1% Aureomycin (ANT), 3) basal diet containing unencapsulated at a dose of 1 × 10 cfu/kg of feed (P1), 4) basal diet containing unencapsulated at a dose of 1 × 10 cfu/kg of feed (P2), 5) basal diet containing 0.01% empty microcapsules (CAP), 6) basal diet containing microencapsulated at a dose of 1 × 10 cfu/kg of feed (CAPP1), and 7) basal diet containing microencapsulated at a dose of 1 × 10 cfu/kg of feed (CAPP2). The feeding experiment included 2 phases: the starter phase from d 1 to 21 and the grower phase from d 22 to 42. The results showed that a 500-L fermenter could produce 20.73 ± 4.05 kg of microcapsules with an approximate diameter of 549 μm. The feeding experiment showed that ADG of broilers in CAPP1 was significantly ( < 0.05) greater than that in CON and CAP throughout the feeding period, whereas the ratio of feed to gain (G:F) was significantly ( < 0.05) lower. Broilers in P1, P2, CAPP1, and CAPP2 had significantly ( < 0.05) greater levels of total superoxide dismutase, catalase, IgG, and cluster of differentiation 3 than those in CON. Furthermore, broilers in CAPP1 had significantly ( < 0.05) greater richness and diversity of intestinal microorganisms, particularly of , than those in all other dietary treatments. In summary, our results indicate that large-scale microencapsulation of microbial cells can be achieved using emulsion and initial gelation and that the dietary administration of microencapsulated can significantly enhance the growth performance, immune function, cecum microbial community, and overall health of broilers.
本研究旨在评估在500升发酵罐中使用乳化和凝胶化方法进行微胶囊中试生产，并评估产品对艾维茵肉鸡生长性能、抗氧化活性、免疫功能和盲肠微生物群的影响。总共700只1日龄雄性艾维茵肉鸡被随机分配到7种日粮处理组，每组5个重复栏，每栏20只肉鸡。日粮处理如下：1）基础日粮（CON），2）含0.1%金霉素的基础日粮（ANT），3）含1×10 cfu/kg饲料未包囊的基础日粮（P1），4）含1×10 cfu/kg饲料未包囊的基础日粮（P2），5）含0.01%空微胶囊的基础日粮（CAP），6）含1×10 cfu/kg饲料微包囊的基础日粮（CAPP1），7）含1×10 cfu/kg饲料微包囊的基础日粮（CAPP2）。饲养试验包括两个阶段：1至21日龄的育雏期和22至42日龄的生长期。结果表明，500升发酵罐可生产约20.73±4.05千克直径约549μm的微胶囊。饲养试验表明，在整个饲养期内，CAPP1组肉鸡的平均日增重（ADG）显著（<0.05）高于CON组和CAP组，而料重比（G:F）显著（<0.05）更低。P1、P2、CAPP1和CAPP2组肉鸡的总超氧化物歧化酶、过氧化氢酶、IgG和分化簇3水平显著（<0.05）高于CON组。此外，CAPP1组肉鸡肠道微生物的丰富度和多样性显著（<0.05）高于所有其他日粮处理组，尤其是[此处原文缺失具体微生物名称]。总之，我们的结果表明，使用乳化和初始凝胶化可以实现微生物细胞的大规模微胶囊化，并且日粮中添加微胶囊化产品可以显著提高肉鸡的生长性能、免疫功能、盲肠微生物群落和整体健康状况。
