Wilson S E, Bourne W M
Department of Ophthalmology, Mayo Clinic Foundation, Rochester, Minnesota.
Surv Ophthalmol. 1989 Jan-Feb;33(4):237-59. doi: 10.1016/0039-6257(82)90150-3.
Significant advances in corneal preservation have been made over the past decade. The introduction of chondroitin sulfate-containing media for use at 4 degrees C allows storage of corneas for up to ten days prior to transplantation. Organ culture techniques have also been improved with the addition of chondroitin sulfate. There has been an increase in our understanding of preservation using McCarey-Kaufman medium. Studies have been published that compare these methods and help the clinician decide which method to use based on objective data. Also, our understanding of existing methods of evaluating endothelial viability has increased and new methods have been developed. Finally, the acquired immunodeficiency syndrome is having an increasing effect on eye-banking and the supply of donor corneas.
在过去十年中,角膜保存技术取得了重大进展。引入含硫酸软骨素的培养基用于4摄氏度保存,使得角膜在移植前可储存长达十天。器官培养技术也因添加硫酸软骨素而得到改进。我们对使用麦卡里 - 考夫曼培养基进行保存的理解有所增加。已发表的研究比较了这些方法,并帮助临床医生根据客观数据决定使用哪种方法。此外,我们对现有评估内皮细胞活力方法的理解有所提高,并且开发了新的方法。最后,获得性免疫缺陷综合征对眼库和供体角膜供应的影响日益增加。