Jiao Haihua, Luo Jinxue, Zhang Yiming, Xu Shengjun, Bai Zhihui, Huang Zhanbin
Changzhi University, Changzhi, Shanxi, China / Key Lab of Environmental Biotec. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China / School of Chemical and Environmental Engineering, China University of Mining and Technology-Beijing, Beijing, China.
Key Lab of Environmental Biotec. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
Pak J Pharm Sci. 2015 Sep;28(5 Suppl):1881-6.
Bio-augmentation is a promising technique for remediation of polluted soils. This study aimed to evaluate the bio-augmentation effect of Rhodobacter sphaeroides biofertilizer (RBF) on the bioremediation of total petroleum hydrocarbons (TPH) contaminated soil. A greenhouse pot experiment was conducted over a period of 120 days, three methods for enhancing bio-augmentation were tested on TPH contaminated soils, including single addition RBF, planting, and combining of RBF and three crop species, such as wheat (W), cabbage (C) and spinach (S), respectively. The results demonstrated that the best removal of TPH from contaminated soil in the RBF bio-augmentation rhizosphere soils was found to be 46.2%, 65.4%, 67.5% for W+RBF, C+RBF, S+RBF rhizosphere soils respectively. RBF supply impacted on the microbial community diversity (phospholipid fatty acids, PLFA) and the activity of soil enzymes, such as dehydrogenase (DH), alkaline phosphatase (AP) and urease (UR). There were significant difference among the soil only containing crude oil (CK), W, C and S rhizosphere soils and RBF bio-augmentation soils. Moreover, the changes were significantly distinct depended on crops species. It was concluded that the RBF is a valuable material for improving effect of remediation of TPH polluted soils.
生物强化是一种很有前景的污染土壤修复技术。本研究旨在评估球形红细菌生物肥料（RBF）对总石油烃（TPH）污染土壤生物修复的生物强化效果。进行了为期120天的温室盆栽试验，分别对TPH污染土壤测试了三种增强生物强化的方法，包括单独添加RBF、种植以及RBF与三种作物（小麦（W）、白菜（C）和菠菜（S））组合。结果表明，在RBF生物强化根际土壤中，TPH从污染土壤中的最佳去除率分别为：W+RBF根际土壤46.2%、C+RBF根际土壤65.4%、S+RBF根际土壤67.5%。RBF的供应影响了微生物群落多样性（磷脂脂肪酸，PLFA）以及土壤酶活性，如脱氢酶（DH）、碱性磷酸酶（AP）和脲酶（UR）。仅含原油的土壤（CK）、W、C和S根际土壤以及RBF生物强化土壤之间存在显著差异。此外，这些变化因作物种类而异。得出的结论是，RBF是提高TPH污染土壤修复效果的一种有价值的材料。
