Ribardière Ambre, Broquet Thomas, Daguin-Thiébaut Claire
CNRS, Station Biologique de Roscoff, Sorbonne Universités, UPMC Univ Paris 06, UMR 7144, Team Diversity and Connectivity of Coastal Marine Landscapes, 29680, Roscoff, France.
BMC Res Notes. 2015 Nov 2;8:632. doi: 10.1186/s13104-015-1595-9.
The Jaera albifrons complex contains five species of marine isopods (J. albifrons, J. praehirsuta, J. ischiosetosa, J. forsmani, and J. posthirsuta). These species, occurring on the shores of the North-Atlantic Ocean, are partially reproductively isolated by barriers due to sexual isolation (mate choice), genetic incompatibilities, and ecological specialization. Microsatellite loci would be useful for parentage-based analyses of sexual selection and studies of genetic structure in the context of speciation.
Twenty-four microsatellite markers were developed for J. albifrons using pyrosequencing of enriched libraries. Patterns of polymorphisms were analyzed in 49 J. albifrons adult males sampled in two populations from Brittany (Western France). The average number of alleles per locus was 4.73 ± 2.45 and the average gene diversity was 0.55 ± 0.23. Most markers also successfully amplified in the three sibling species J. praehirsuta, J. ischiosetosa, and J. forsmani.
These polymorphic and cross-amplifiable markers will be useful for population genetics and parentage studies in the J albifrons complex.
白额耶尔虾复合体包含五种海洋等足类动物（白额耶尔虾、前刚毛耶尔虾、坐骨刚毛耶尔虾、福氏耶尔虾和后刚毛耶尔虾）。这些物种分布在北大西洋沿岸，由于性隔离（配偶选择）、遗传不相容性和生态特化等障碍，它们在一定程度上存在生殖隔离。微卫星基因座对于基于亲子关系的性选择分析以及物种形成背景下的遗传结构研究将是有用的。
利用富集文库的焦磷酸测序技术为白额耶尔虾开发了24个微卫星标记。对从法国西部布列塔尼的两个种群中采集的49只白额耶尔虾成年雄性个体的多态性模式进行了分析。每个基因座的平均等位基因数为4.73±2.45，平均基因多样性为0.55±0.23。大多数标记在三个近缘物种前刚毛耶尔虾、坐骨刚毛耶尔虾和福氏耶尔虾中也成功扩增。
这些多态性且可交叉扩增的标记将对白额耶尔虾复合体的群体遗传学和亲子关系研究有用。
