一种基于聚多巴胺纳米球的具有酶放大作用的高灵敏度和选择性适体细胞传感器。
A polydopamine nanosphere based highly sensitive and selective aptamer cytosensor with enzyme amplification.
作者信息
Fan Daoqing, Wu Changtong, Wang Kun, Gu Xiaoxiao, Liu Yaqing, Wang Erkang
机构信息
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, P. R. China.
出版信息
Chem Commun (Camb). 2016 Jan 7;52(2):406-9. doi: 10.1039/c5cc06754e. Epub 2015 Nov 3.
With CCRF-CEM as the model cell, a highly sensitive and selective cytosensor was developed by taking advantage of polydopamine nanospheres for the first time. The strategies of aptamer/membrane protein recognition and Exonuclease III assisted cycle amplification were used for improving selectivity and sensitivity. The detection of limit reached was as low as 15 cells per mL.
以CCRF-CEM为模型细胞，首次利用聚多巴胺纳米球开发了一种高灵敏度和高选择性的细胞传感器。采用适体/膜蛋白识别和核酸外切酶III辅助循环扩增策略提高选择性和灵敏度。达到的检测限低至每毫升15个细胞。
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