Chemistry Department, Faculty of Sciences and Technology, University of Coimbra, 3004-535, Coimbra, Portugal.
Serviços Farmacêuticos, Centro Hospitalar e Universitário de Coimbra, EPE, 3000-075 Coimbra, Portugal.
Anal Chim Acta. 2015 Oct 22;898:28-33. doi: 10.1016/j.aca.2015.09.049. Epub 2015 Oct 8.
The interaction of the anticancer monoclonal antibody bevacizumab (BEVA) with double-stranded DNA (dsDNA) was studied by voltammetry and gel-electrophoresis in incubated samples and using the dsDNA-electrochemical biosensor. The voltammetric results revealed a decrease and disappearance of the dsDNA oxidation peaks with increasing incubation time, showing that BEVA binds to the dsDNA but no DNA oxidative damage was detected electrochemically. Non denaturing agarose gel-electrophoresis experiments were in agreement with the voltammetric results showing the formation of compact BEVA-dsDNA adduct. The dsDNA-electrochemical biosensor in incubated solutions showed that BEVA also undergoes structural modification upon binding dsDNA, and BEVA electroactive amino acid residues oxidation peaks were detected.
通过伏安法和凝胶电泳研究了抗癌单克隆抗体贝伐珠单抗（BEVA）与双链 DNA（dsDNA）的相互作用，在孵育样本中以及使用 dsDNA 电化学生物传感器进行了研究。伏安法结果表明，随着孵育时间的增加，dsDNA 氧化峰的降低和消失，表明 BEVA 与 dsDNA 结合，但电化学上未检测到 DNA 氧化损伤。非变性琼脂糖凝胶电泳实验与伏安法结果一致，表明形成了紧凑的 BEVA-dsDNA 加合物。孵育溶液中的 dsDNA 电化学生物传感器表明，BEVA 在与 dsDNA 结合时也会发生结构修饰，并检测到 BEVA 电化学活性氨基酸残基的氧化峰。
