Department of Chemistry, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran.
Department of Chemistry, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran.
Anal Chim Acta. 2015 Oct 22;898:42-9. doi: 10.1016/j.aca.2015.09.052. Epub 2015 Oct 9.
In the present research, an effective on chip electromembrane extraction (CEME) coupled with high performance liquid chromatography was presented for analysis of nortriptyline (NOR) and amitriptyline (AMI) as basic model analytes from urine samples. The chip consists of two polymethyl methacrylate (PMMA) parts with two craved microfluidic channels in each part. These channels were used as flow path for the sample solution and a thin compartment for the acceptor phase. A porous polypropylene sheet membrane impregnated with an organic solvent was placed between two parts of chip device to separate the channels. Two platinum electrodes were mounted at the bottom of these channels that were connected to a power supply providing the electrical driving force for migration of ionized analytes from sample solution through the porous sheet membrane into the acceptor phase. This new setup provides effective and reproducible extractions with low volume of sample solution. Efficient parameters on CEME of the model analytes were optimized using one variable at a time method. Under the optimized conditions, the calibration curve was linear in the range of 10.0-500 μg L(-1) with coefficient of determination (r(2)) more than 0.9902. The relative standard deviations (RSDs %) for extraction and determination of the analytes were less than 6.8% based on six replicate measurements. LODs less than 4.0 μg L(-1) were obtained for both of the model analytes. The preconcentration factors higher than 17.0-fold were obtained. The results demonstrated that CEME would be used efficiently for extraction and determination of AMI and NOR from urine samples.
在本研究中，提出了一种有效的片上电膜萃取（CEME）与高效液相色谱法相结合，用于分析尿液样品中的去甲替林（NOR）和阿米替林（AMI）作为基本模型分析物。该芯片由两个聚甲基丙烯酸甲酯（PMMA）部分组成，每个部分都有两个雕刻的微流道。这些通道用作样品溶液的流道和薄的接受相室。在芯片装置的两个部分之间放置了一个浸渍有机溶剂的多孔聚丙烯片膜，以分离通道。两个铂电极安装在这些通道的底部，与电源连接，为带电荷的分析物从样品溶液通过多孔片膜迁移到接受相提供电驱动力。这种新的设置提供了有效的和可重复的提取，使用的样品溶液体积低。使用单变量法优化了模型分析物的 CEME 的有效参数。在优化条件下，校准曲线在 10.0-500μg/L 的范围内呈线性，相关系数（r(2)）大于 0.9902。基于六个重复测量，分析物的提取和测定的相对标准偏差（RSD%）小于 6.8%。两种模型分析物的检出限（LOD）均小于 4.0μg/L。获得了高于 17.0 倍的浓缩因子。结果表明，CEME 将有效地用于从尿液样品中提取和测定 AMI 和 NOR。
