早期发育过程中心外膜作为心脏自主神经反应的调节因子。

The epicardium as modulator of the cardiac autonomic response during early development.

作者信息

Kelder Tim P, Duim Sjoerd N, Vicente-Steijn Rebecca, Végh Anna M D, Kruithof Boudewijn P T, Smits Anke M, van Bavel Thomas C, Bax Noortje A M, Schalij Martin J, Gittenberger-de Groot Adriana C, DeRuiter Marco C, Goumans Marie-José, Jongbloed Monique R M

机构信息

Department of Anatomy & Embryology, Leiden University Medical Center, Leiden, The Netherlands.

Department of Molecular Cell Biology, Leiden University Medical Center, Leiden, The Netherlands.

出版信息

J Mol Cell Cardiol. 2015 Dec;89(Pt B):251-9. doi: 10.1016/j.yjmcc.2015.10.025. Epub 2015 Oct 23.

DOI:10.1016/j.yjmcc.2015.10.025

PMID:26527381

Abstract

The cardiac autonomic nervous system (cANS) modulates heart rate, contraction force and conduction velocity. The embryonic chicken heart already responds to epinephrine prior to establishment of the cANS. The aim of this study was to define the regions of the heart that might participate in modulating the early autonomic response to epinephrine. Immunofluorescence analysis reveals expression of neural markers tubulin beta-3 chain and neural cell adhesion molecule in the epicardium during early development. In addition, expression of the β2 adrenergic receptor, the receptor for epinephrine, was found in the epicardium. Ex-ovo micro-electrode recordings in hearts with inhibition of epicardial outgrowth showed a significantly reduced response of the heart rate to epinephrine compared to control hearts. This study suggests a role for the epicardium as autonomic modulator during early cardiac development.

摘要

心脏自主神经系统（cANS）调节心率、收缩力和传导速度。在心脏自主神经系统建立之前，胚胎期的鸡心脏就已经对肾上腺素产生反应。本研究的目的是确定心脏中可能参与调节对肾上腺素早期自主反应的区域。免疫荧光分析显示，在早期发育过程中，心外膜中有神经标志物微管蛋白β-3链和神经细胞黏附分子的表达。此外，在心外膜中发现了肾上腺素的受体β2肾上腺素能受体的表达。与对照心脏相比，在心外膜生长受到抑制的心脏中进行的体外微电极记录显示，心率对肾上腺素的反应明显降低。这项研究表明，在心早期发育过程中，心外膜作为自主调节因子发挥作用。