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蜡样芽孢杆菌外排蛋白BC3310——未知主要易化子家族UMF-2的一种多药转运蛋白。

Bacillus cereus efflux protein BC3310 - a multidrug transporter of the unknown major facilitator family, UMF-2.

作者信息

Kroeger Jasmin K, Hassan Karl, Vörös Aniko, Simm Roger, Saidijam Massoud, Bettaney Kim E, Bechthold Andreas, Paulsen Ian T, Henderson Peter J F, Kolstø Anne-Brit

机构信息

Laboratory for Microbial Dynamics, Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo Oslo, Norway ; Institut für Pharmazeutische Biologie und Biotechnologie, Albert-Ludwigs Universität Freiburg, Germany.

Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney NSW, Australia.

出版信息

Front Microbiol. 2015 Oct 12;6:1063. doi: 10.3389/fmicb.2015.01063. eCollection 2015.

DOI:10.3389/fmicb.2015.01063
PMID:26528249
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4601019/
Abstract

Phylogenetic classification divides the major facilitator superfamily (MFS) into 82 families, including 25 families that are comprised of transporters with no characterized functions. This study describes functional data for BC3310 from Bacillus cereus ATCC 14579, a member of the "unknown major facilitator family-2" (UMF-2). BC3310 was shown to be a multidrug efflux pump conferring resistance to ethidium bromide, SDS and silver nitrate when heterologously expressed in Escherichia coli DH5α ΔacrAB. A conserved aspartate residue (D105) in putative transmembrane helix 4 was identified, which was essential for the energy dependent ethidium bromide efflux by BC3310. Transport proteins of the MFS comprise specific sequence motifs. Sequence analysis of UMF-2 proteins revealed that they carry a variant of the MFS motif A, which may be used as a marker to distinguish easily between this family and other MFS proteins. Genes orthologous to bc3310 are highly conserved within the B. cereus group of organisms and thus belong to the core genome, suggesting an important conserved functional role in the normal physiology of these bacteria.

摘要

系统发育分类将主要易化子超家族（MFS）分为82个家族，其中包括25个由功能未明确的转运蛋白组成的家族。本研究描述了蜡样芽孢杆菌ATCC 14579中BC3310的功能数据，BC3310是“未知主要易化子家族-2”（UMF-2）的成员。当在大肠杆菌DH5α ΔacrAB中异源表达时，BC3310被证明是一种多药外排泵，可赋予对溴化乙锭、十二烷基硫酸钠和硝酸银的抗性。在假定的跨膜螺旋4中鉴定出一个保守的天冬氨酸残基（D105），它对于BC3310依赖能量的溴化乙锭外排至关重要。MFS的转运蛋白包含特定的序列基序。对UMF-2蛋白的序列分析表明，它们携带MFS基序A的变体，这可作为一个标记，用于轻松区分该家族与其他MFS蛋白。与bc3310直系同源的基因在蜡样芽孢杆菌生物群中高度保守，因此属于核心基因组，这表明它们在这些细菌的正常生理学中具有重要的保守功能作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11a1/4601019/81c3854e49ac/fmicb-06-01063-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11a1/4601019/e259bc305056/fmicb-06-01063-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11a1/4601019/9d7c13955649/fmicb-06-01063-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11a1/4601019/6ef980c6f859/fmicb-06-01063-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11a1/4601019/be7b1b2f9c3b/fmicb-06-01063-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11a1/4601019/5b95551598b9/fmicb-06-01063-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11a1/4601019/81c3854e49ac/fmicb-06-01063-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11a1/4601019/e259bc305056/fmicb-06-01063-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11a1/4601019/9d7c13955649/fmicb-06-01063-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11a1/4601019/6ef980c6f859/fmicb-06-01063-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11a1/4601019/be7b1b2f9c3b/fmicb-06-01063-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11a1/4601019/5b95551598b9/fmicb-06-01063-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/11a1/4601019/81c3854e49ac/fmicb-06-01063-g006.jpg

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