Welch Elaine, Pelegri Francisco
a Laboratory of Genetics; University of Wisconsin - Madison ; Madison , WI USA.
Bioarchitecture. 2014;5(1-2):13-26. doi: 10.1080/19490992.2015.1080891.
In zebrafish embryos, factors involved in both axis induction and primordial germ cell (PGC) development are localized to the vegetal pole of the egg. However, upon egg activation axis induction factors experience an asymmetric off-center shift whereas PGC factors undergo symmetric animally-directed movement. We examined the spatial relationship between the proposed dorsal genes wnt8a and grip2a and the PGC factor dazl at the vegetal cortex. We find that RNAs for these genes localize to different cortical depths, with the RNA for the PGC factor dazl at a deeper cortical level than those for axis-inducing factors. In addition, and in contrast to the role of microtubules in the long-range transport of dorsal determinants, we find that germ line determinant transport depends on the actin cytoskeleton. Our results support a model in which vegetal cortex differential RNA transport behavior is facilitated by RNA localization along cortical depth and differential coupling to cortical transport.
在斑马鱼胚胎中，参与轴诱导和原始生殖细胞（PGC）发育的因子定位于卵的植物极。然而，在卵激活后，轴诱导因子经历不对称的偏心移位，而PGC因子则进行对称的向动物极方向的移动。我们研究了拟议的背侧基因wnt8a和grip2a与植物皮质处的PGC因子dazl之间的空间关系。我们发现这些基因的RNA定位于不同的皮质深度，PGC因子dazl的RNA位于比轴诱导因子的RNA更深的皮质水平。此外，与微管在背侧决定因子的长距离运输中的作用相反，我们发现生殖系决定因子的运输依赖于肌动蛋白细胞骨架。我们的结果支持一种模型，即植物皮质差异RNA运输行为是由沿皮质深度的RNA定位和与皮质运输的差异偶联促进的。
