1 State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University , Beijing, China .
2 College of Life Sciences, Zhejiang Sci-Tech University , Hangzhou, China .
Tissue Eng Part A. 2016 Jan;22(1-2):170-81. doi: 10.1089/ten.tea.2015.0237. Epub 2015 Dec 17.
In this study, human mesenchymal stem cells (hMSCs) were cultured on the hydroxyapatite (HA) and mineralized collagen (MC), and their proliferation, adhesion, and differentiation, especially the molecular mechanisms on gene level, were investigated. Proliferation and morphological responses of hMSCs and their osteogenic differentiation were detected by quantitative detection of alkaline phosphatase. Gene expression profilings were examined by microarrays, and the gene expression data were studied through gene ontology terms and pathway analyses. The results showed that MC promoted cell proliferation and osteogenic differentiation of hMSCs. Microarray analysis showed that MC was conducive to express osteogenesis-related genes, such as BMP-2, COL1A1, and CTSK, and stimulate osteogenic differentiation, such as osteoblast differentiation pathway and skeletal system development pathway.
在这项研究中，将人骨髓间充质干细胞（hMSCs）培养在羟基磷灰石（HA）和矿化胶原（MC）上，研究了它们的增殖、黏附和分化，特别是在基因水平上的分子机制。通过碱性磷酸酶的定量检测来检测 hMSCs 的增殖和形态反应及其成骨分化。通过微阵列检查基因表达谱，并通过基因本体术语和途径分析研究基因表达数据。结果表明，MC 促进了 hMSCs 的增殖和成骨分化。微阵列分析表明，MC 有利于表达成骨相关基因，如 BMP-2、COL1A1 和 CTSK，并刺激成骨分化，如成骨细胞分化途径和骨骼系统发育途径。
