van Ewijk Petronella A, Paglialunga Sabina, Kooi M Eline, Nunes Patricia M, Gemmink Anne, Slenter Jos, Kornips Esther, Jörgensen Johanna A, Hoeks Joris, Wildberger Joachim E, Hesselink Matthijs K C, Glatz Jan F C, Heerschap Arend, Kersten Sander, Schrauwen Patrick, Schrauwen-Hinderling Vera B
Department of Human Biology, Maastricht University, Maastricht, The Netherlands.
Department of Radiology, Maastricht University Hospital, Maastricht, The Netherlands.
Obesity (Silver Spring). 2015 Nov;23(11):2242-50. doi: 10.1002/oby.21235.
Parental high-fat feeding was proposed to negatively impact metabolic health in offspring. Here, the ectopic fat storage in heart and liver in offspring was investigated, and the effects on mitochondrial function, de novo lipogenesis, and postprandial lipid metabolism were explored in detail.
Male and female mice received either a high-fat (HF) or standard chow (LF) diet during mating, gestation and lactation. All offspring animals received the HF diet.
Abdominal visceral adipose tissue tended to be higher in HF/HF mice. Cardiac lipid content was also higher in the HF/HF mice (LF/HF vs.
HF/HF: 1.03% ± 0.08% vs. 1.33% ± 0.07% of water signal, P = 0.01). In contrast, hepatic lipid content tended to be lower in HF/HF mice compared to LF/HF mice. A severely disturbed postprandial lipid clearance was revealed in HF/HF mice by the results from the triglyceride (TG) tolerance tests (LF/HF vs.
HF/HF: 6,753 ± 2,213 vs. 14,367 ± 1,978 mmol l(-1) min(-1) , P = 0.01) and (13) C-fatty acid retention test (LF/HF vs.
HF/HF: 2.73% ± 0.85% vs. 0.89% ± 0.26% retention from bolus, P = 0.04), which may underlie the lower hepatic lipid content.
Here it is shown that HF diet negatively impacts postprandial TG clearance in offspring and results in an overall metabolic unfavorable phenotype and ectopic lipid deposition in the heart and in visceral storage sites.
有人提出，亲代高脂喂养会对后代的代谢健康产生负面影响。在此，我们研究了后代心脏和肝脏中的异位脂肪储存，并详细探讨了其对线粒体功能、从头脂肪生成和餐后脂质代谢的影响。
雄性和雌性小鼠在交配、妊娠和哺乳期接受高脂（HF）或标准饲料（LF）饮食。所有后代动物均接受高脂饮食。
HF/HF小鼠的腹部内脏脂肪组织往往更高。HF/HF小鼠的心脏脂质含量也更高（LF/HF与HF/HF：水信号的1.03%±0.08%与1.33%±0.07%，P=0.01）。相比之下，与LF/HF小鼠相比，HF/HF小鼠的肝脏脂质含量往往更低。甘油三酯（TG）耐量试验结果显示，HF/HF小鼠的餐后脂质清除严重受损（LF/HF与HF/HF：6,753±2,213与14,367±1,978 mmol l(-1) min(-1)，P=0.01）以及（13）C-脂肪酸保留试验（LF/HF与HF/HF：推注后保留率2.73%±0.85%与0.89%±0.26%，P=0.04），这可能是肝脏脂质含量较低的原因。
本研究表明，高脂饮食会对后代的餐后TG清除产生负面影响，并导致整体代谢不良表型以及心脏和内脏储存部位的异位脂质沉积。
