Volm M, Efferth T, Bak M, Mattern J
Department of Experimental Pathology, German Cancer Research Center, Heidelberg, FRG.
Arch Gynecol Obstet. 1989;244(2):123-8. doi: 10.1007/BF00931383.
Drug-resistant cancer cells with the multidrug-resistance phenotype show overexpression of P-glycoprotein, and we therefore tested carcinoma tissue from five patients with stage III or IV ovarian cancer for P-glycoprotein using 265/F4 and C 219 monoclonal antibodies, prepared against membrane glycoproteins in colchicine-resistant CHO cells. Using immunofluorescence and immunoblotting techniques, one of the tumors showed a positive reaction. Using the pcDR 1.5 clone we found that the same cancer tissue had elevated expression of the genes responsible for multidrug resistance. The demonstration of elevated P-glycoprotein in ovarian carcinomas indicates that P-glycoprotein overexpression is not limited to experimental tumor models.
具有多药耐药表型的耐药癌细胞显示P-糖蛋白过表达，因此我们使用针对耐秋水仙碱的中国仓鼠卵巢细胞（CHO）中的膜糖蛋白制备的265/F4和C 219单克隆抗体，检测了五名III期或IV期卵巢癌患者的癌组织中的P-糖蛋白。使用免疫荧光和免疫印迹技术，其中一个肿瘤显示出阳性反应。使用pcDR 1.5克隆，我们发现同一癌组织中负责多药耐药的基因表达升高。卵巢癌中P-糖蛋白升高的证明表明，P-糖蛋白过表达并不局限于实验性肿瘤模型。
