König Karsten, Andersen Peter, Le Tuan, Breunig Hans Georg
JenLab GmbH, Jena, D-07745, Germany.
Department of Biophotonics and Laser Technology, Saarland University, Saarbrücken, D-66123, Germany.
Microsc Res Tech. 2015 Dec;78(12):1154-8. doi: 10.1002/jemt.22599. Epub 2015 Nov 4.
Multiphoton laser scanning microscopy commonly relies on bulky and expensive femtosecond lasers. We integrated a novel minimal-footprint Ti:sapphire oscillator, pumped by a frequency-doubled distributed Bragg reflector tapered diode laser, into a clinical multiphoton tomograph and evaluated its imaging capability using different biological samples, i.e. cell monolayers, corneal tissue, and human skin. With the novel laser, the realization of very compact Ti:sapphire-based systems for high-quality multiphoton imaging at a significantly size and weight compared to current systems will become possible.
