Sun P, Gu C Y, Ding Y Y, Wei J H, Yin Z J, Geng Z Y, Li Y
Veterinary Department, College of Animal Science and Technology, Technology Research Institute of Industrial Genetics of Livestock and Poultry in Anhui Province, Anhui Agricultural University, Anhui, China.
Anhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Animal Science Department, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui, China.
Genet Mol Res. 2015 Oct 28;14(4):13403-13. doi: 10.4238/2015.October.28.2.
To assess the homology and phylogenetic relationship between porcine epidemic diarrhea virus (PEDV) Anhui strains and other PEDV strains, molecular homology and phylogenetic analyses of Anhui PEDV field strains were compared with those of reference strains. The results revealed that the M and N genes of PEDV were 681 and 1326 bp long, respectively. The nucleotide sequences of the N genes of Anhui PEDV strains were 95.9-99.9% homologous with each other, and the deduced amino acid sequences were 92.5-99.8% homologous. Compared with the PEDV reference strains, the Anhui PEDV field strains had 94.1-99.5% nucleotide sequence homology in the N gene and 91.2-97.5% amino acid mutation homology in the N protein. The nucleotide sequences of the M genes of Anhui PEDV were 98.3-100% homologous, and the deduced amino acid sequences were 96.5-99.6% homologous. Compared with the PEDV reference strains, the Anhui PEDV field strains had 96.9-100% nucleotide sequence homology in the M gene and 96.5-99.6% amino acid homology in the M protein. The Anhui strains were genetically similar to USA strains (USA/Iowa/16465/2013 and USA/Indiana/17846/2013) but different from European (CV777;Br1/7), Korean (Chinju99), and Japanese (83p-5) strains.
为评估猪流行性腹泻病毒（PEDV）安徽株与其他PEDV毒株之间的同源性和系统发育关系，将安徽PEDV田间毒株与参考毒株进行了分子同源性和系统发育分析。结果显示，PEDV的M基因和N基因长度分别为681 bp和1326 bp。安徽PEDV毒株N基因的核苷酸序列相互间同源性为95.9% - 99.9%，推导的氨基酸序列同源性为92.5% - 99.8%。与PEDV参考毒株相比，安徽PEDV田间毒株N基因的核苷酸序列同源性为94.1% - 99.5%，N蛋白的氨基酸变异同源性为91.2% - 97.5%。安徽PEDV的M基因核苷酸序列同源性为98.3% - 100%，推导的氨基酸序列同源性为96.5% - 99.6%。与PEDV参考毒株相比，安徽PEDV田间毒株M基因的核苷酸序列同源性为96.9% - 100%，M蛋白的氨基酸同源性为96.5% - 99.6%。安徽毒株在基因上与美国毒株（USA/Iowa/16465/2013和USA/Indiana/17846/2013）相似，但与欧洲毒株（CV777；Br1/7）、韩国毒株（Chinju99）和日本毒株（83p - 5）不同。
