Kuken B N, Aikemu A N W E, Xiang S Y, Wulasihan M H Y T
Department of Cardiology, The Second Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
Department of Drug Analysis, Faculty of Pharmacy, Xinjiang Medical University, Urumqi, Xinjiang, China.
Genet Mol Res. 2015 Oct 29;14(4):13625-32. doi: 10.4238/2015.October.28.24.
To study the effects of overexpression of the sarcoplasmic reticulum ATPase 2a (SERCA2a) gene on the activity and protein expression of SERCA2a after rapid atrial pacing (RAP) in New Zealand white rabbits. New Zealand white rabbits were randomly divided into a sham-operated group (group A), adeno-associated virus 1 (AAV1)/EGFP + atrial fibrillation (AF) model group (group B), or AVV1/SERCA2a + AF group (group C). The sham-operated group was used as a negative control. Each group consisted of 10 animals. Groups B and C were injected with 500 μL of the AAV1-EGFP reporter gene and 500 μL of the AAV1-SERCA2a target gene, respectively. Four weeks after AAV1-mediated gene transfer, the rabbits underwent 24 h of RAP to the right atrium. The animals were sacrificed and protein activity and protein expression in the myocardium were measured using the westernblot method. Four weeks after AAV1-mediated gene transfer, SERCA2a protein activity and expression were significantly higher in Group C than in Groups A and B (P < 0.05). RAP of the right atrium induced atrial fibrillation in rabbits, resulting in decreases in the activity and protein expression of SERCA2a. Pericardial AAV-1 mediated SERCA2a gene transfer resulted in the overexpression of SERCA2a, restoring SERCA2a activity and protein expression.
研究肌浆网ATP酶2a（SERCA2a）基因过表达对新西兰白兔快速心房起搏（RAP）后SERCA2a活性及蛋白表达的影响。将新西兰白兔随机分为假手术组（A组）、腺相关病毒1（AAV1）/增强绿色荧光蛋白（EGFP）+心房颤动（AF）模型组（B组）或AAV1/SERCA2a + AF组（C组）。假手术组作为阴性对照。每组10只动物。B组和C组分别注射500 μL的AAV1 - EGFP报告基因和500 μL的AAV1 - SERCA2a靶基因。在AAV1介导的基因转移4周后，对兔子右心房进行24小时的RAP。处死动物，采用蛋白质印迹法检测心肌中的蛋白活性和蛋白表达。在AAV1介导的基因转移4周后，C组的SERCA2a蛋白活性和表达显著高于A组和B组（P < 0.05）。右心房RAP诱导兔子发生心房颤动，导致SERCA2a活性和蛋白表达降低。心包内AAV - 1介导的SERCA2a基因转移导致SERCA2a过表达，恢复了SERCA2a活性和蛋白表达。
