Chwiej J, Patulska A, Skoczen A, Janeczko K, Ciarach M, Simon R, Setkowicz Z
Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, Krakow, Poland.
Institute of Zoology, Jagiellonian University, Krakow, Poland.
J Biol Inorg Chem. 2015 Dec;20(8):1277-86. doi: 10.1007/s00775-015-1306-y. Epub 2015 Nov 4.
The main purpose of the following study was the determination of elemental changes occurring within hippocampal formation as a result of high-fat and carbohydrate-restricted ketogenic diet (KD). To realize it, X-ray fluorescence microscopy was applied for topographic and quantitative analysis of P, S, K, Ca, Fe, Cu, Zn and Se in hippocampal formations taken from rats fed with two different KDs and naive controls. The detailed comparisons were done for sectors 1 and 3 of the Ammon's, the dentate gyrus and hilus of dentate gyrus. The results of elemental analysis showed that the KDs induced statistically significant changes in the accumulation of P, K, Ca, Zn and Se in particular areas of hippocampal formation and these alterations strongly depended on the composition of the diets. Much greater influence on the hippocampal areal densities of examined elements was found for the KD which was characterized by a lower content of carbohydrates, higher content of fats and increased proportion of unsaturated fatty acids. The levels of P, K and Zn decreased whilst those of Ca and Se increased as a result of the treatment with the KDs.
以下研究的主要目的是确定高脂和低碳水化合物生酮饮食（KD）导致海马结构内发生的元素变化。为实现这一目的，采用X射线荧光显微镜对取自喂食两种不同KD的大鼠及未处理对照组的海马结构中的磷（P）、硫（S）、钾（K）、钙（Ca）、铁（Fe）、铜（Cu）、锌（Zn）和硒（Se）进行地形和定量分析。对海马的第1和第3扇区、齿状回以及齿状回门进行了详细比较。元素分析结果表明，KD在海马结构的特定区域引起了磷、钾、钙、锌和硒积累的统计学显著变化，且这些改变很大程度上取决于饮食组成。对于碳水化合物含量较低、脂肪含量较高且不饱和脂肪酸比例增加的KD，发现其对所检测元素在海马区域密度的影响更大。经KD处理后，磷、钾和锌的水平降低，而钙和硒的水平升高。
