Versura P, Cellini M, Zucchini P G, Caramazza R, Laschi R
Institute of Clinical Electron Microscopy, University of Bologna, Italy.
Cornea. 1989;8(2):81-9.
The effect of topically administered cyclosporin A (CyA) was evaluated in this experimental study by means of transmission and scanning electron microscopy, and immunohistochemistry. CyA dissolved in sterile olive oil was injected in the retrobulbar area and in the subconjunctival space, or administered as eyedrops in both non-grafted and corneal xenografted rabbit eyes. The vehicle rather than the drug was thought to be responsible for the inflammatory reaction observed in the conjunctiva of the injected eyes as well as for the surface epithelial defects developing in the cornea of the topically administered eyes. CyA was found to be effective in prolonging the survival of the xenografts after 1 month follow-up. The immunohistochemistry indirectly demonstrated the CyA effectiveness in impairing the T-cell function, and this is, to our knowledge, the first documented report on this subject. We conclude that another type of vehicle for the drug has to be introduced and that the administration of CyA as eyedrops is the best route to provide fast diffusion and to avoid adverse side effects.
在本实验研究中，通过透射电子显微镜、扫描电子显微镜和免疫组织化学方法评估了局部应用环孢素A（CyA）的效果。将溶解于无菌橄榄油中的CyA注射到兔眼的球后区域和结膜下间隙，或在未移植和角膜异种移植的兔眼中滴眼给药。人们认为，引起注射眼结膜炎症反应以及滴眼给药眼角膜表面上皮缺损的原因是赋形剂而非药物。随访1个月后发现，CyA可有效延长异种移植物的存活时间。免疫组织化学间接证明了CyA在损害T细胞功能方面的有效性，据我们所知，这是关于该主题的首篇有文献记载的报告。我们得出结论，必须引入另一种药物赋形剂，且滴眼给药是使CyA快速扩散并避免不良副作用的最佳途径。
