Tian Xiaohe, Wang Hui, Guan Lijuan, Zhang Qiong, Zhou Hongping, Li Chunxia, Huang Bei, Wu Jieying, Tian Yupeng
School of Life Science, Anhui University, Hefei, 230601, People's Republic of China.
Department of Chemistry, Key Laboratory of Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei, 230601, People's Republic of China.
J Fluoresc. 2016 Jan;26(1):59-65. doi: 10.1007/s10895-015-1703-1. Epub 2015 Nov 4.
In this letter, aggregation from two-photon absorption (2PA) molecules in living cells were firstly observed and the related aggregation induced emission (AIE) properties were investigated as a cell tracer for L ((Z)-3-(4-(Bis(4-ethoxyphenyl) amino)phenyl)-2-(4-amino-phenyl)- acrylonitrile cyano-substituted ) based on triphenylamine with D-π-A model. L was further used as a two-photon absorption (2PA, λex = 900, λem = 550 nm δ = 156 GM) live-cell marker for real-time, long-term cell growth and proliferation monitoring, with rapidly adhering whole intracellular membrane-rich system. Remarkably, different from existing organic AIE chromophores and other commercially available probes, L exhibited intense intracellular-AIE property with stable nuclear envelope (NE) staining under two-photon excited microscopy (TPEM) through detailed in cellulo studies.
在这封信中，首次观察到活细胞中双光子吸收（2PA）分子的聚集现象，并基于具有D-π-A模型的三苯胺对相关的聚集诱导发光（AIE）特性进行了研究，该特性作为基于L（（Z）-3-（4-（双（4-乙氧基苯基）氨基）苯基）-2-（4-氨基苯基）-丙烯腈氰基取代）的细胞示踪剂。L进一步用作双光子吸收（2PA，λex = 900，λem = 550 nm，δ = 156 GM）活细胞标记物，用于实时、长期监测细胞生长和增殖，具有快速粘附的富含整个细胞内膜的系统。值得注意的是，与现有的有机AIE发色团和其他市售探针不同，通过详细的细胞内研究，L在双光子激发显微镜（TPEM）下表现出强烈的细胞内AIE特性，并具有稳定的核膜（NE）染色。
