Tomiita Minako, Campos-Alberto Eduardo, Shima Masayuki, Namiki Masanobu, Sugimoto Kazuo, Kojima Hiroyuki, Watanabe Hiroko, Sekine Kunio, Nishimuta Toshiyuki, Kohno Yoichi, Shimojo Naoki
Department of Pediatrics, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
Department of Public Health, Hyogo College of Medicine, Nishinomiya, Hyogo Prefecture 663-8131, Japan.
Asia Pac Allergy. 2015 Oct;5(4):210-5. doi: 10.5415/apallergy.2015.5.4.210. Epub 2015 Oct 28.
The immunological mechanisms of asthma remission remain unclear although several reports have suggested that balance between T helper (Th) 2 cytokines and regulatory cytokines is related.
To study the balance between interleukin (IL) 10 and IL-5 in asthma clinical remission.
We measured the numbers of IL-5 and IL-10 producing cells in peripheral blood mononuclear cells stimulated with mite antigen obtained from patients with active asthma (group A, n = 18), patients in clinical remission (group R, n = 15) and nonatopic healthy controls (group H, n = 14).
The numbers of IL-5 producing cells in groups A and R were significantly higher than in group H. The number of IL-5 producing cells was lower in group R than in group A, although the difference was not statistically significant. The number of IL-10 producing cells was higher in group R than in group A, although again the difference was not statistically significant. There was a significant difference in the number of IL-10 producing cells between groups A and H but not between groups R and H. The ratio of the number of IL-10 to IL-5 producing cells was highest in group H followed by groups R and A, and the differences were statistically significant for each pair of groups.
Our study suggests that the IL-10/IL-5 balance is related to clinical asthma. The balance differs between patients in clinical remission and healthy controls, suggesting that allergic inflammation may continue even after clinical asthma remission.
尽管有几份报告表明辅助性T（Th）2细胞因子与调节性细胞因子之间的平衡有关，但哮喘缓解的免疫机制仍不清楚。
研究哮喘临床缓解期白细胞介素（IL）-10与IL-5之间的平衡。
我们检测了来自活动期哮喘患者（A组，n = 18）、临床缓解期患者（R组，n = 15）和非特应性健康对照者（H组，n = 14）的外周血单个核细胞经螨抗原刺激后产生IL-5和IL-10的细胞数量。
A组和R组中产生IL-5的细胞数量显著高于H组。R组中产生IL-5的细胞数量低于A组，尽管差异无统计学意义。R组中产生IL-10的细胞数量高于A组，同样差异无统计学意义。A组和H组之间产生IL-10的细胞数量有显著差异，但R组和H组之间无显著差异。产生IL-10的细胞数量与产生IL-5的细胞数量之比在H组最高，其次是R组和A组，且每组之间的差异均有统计学意义。
我们的研究表明IL-10/IL-5平衡与临床哮喘有关。临床缓解期患者与健康对照者之间的平衡不同，这表明即使在临床哮喘缓解后，过敏性炎症可能仍会持续。
