Kwon Jeong Heo, Lee Siseon, Lee Jae-Won, Hong Youn-Woo, Chang Jeong Ho, Sung Daekyung, Kim Sung Hyun, Sang Byoung-In, Mitchell Robert J, Lee Jin Hyung
Korea Institute of Ceramic Engineering and Technology (KICET), 101 Soho-ro, Jinju-si, Gyeongsangnam-do 52851, Republic of Korea ; Department of Chemical Engineer, Hanyang University, Seoul 04763, Republic of Korea.
School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan 44919, Republic of Korea.
Biomed Res Int. 2015;2015:267538. doi: 10.1155/2015/267538. Epub 2015 Oct 11.
This paper describes an optimization of planetary mill pretreatment and saccharification processes for improving biosugar production. Pitch pine (Pinus rigida) wood sawdust waste was used as biomass feedstock and the process parameters optimized in this study were the buffering media, the milling time, the enzyme quantity, and the incubation time. Glucose yields were improved when acetate buffer was used rather than citrate buffer. Initially, with each process variable tests, the optimal values were 100 minutes of milling, an enzyme concentration of 16 FPU/g-biomass, and a 12-hour enzymatic hydrolysis. Typically, interactions between these experimental conditions and their effects on glucose production were next investigated using RSM. Glucose yields from the Pinus rigida waste exceeded 80% with several of the conditions tested, demonstrating that milling can be used to obtain high levels of glucose bioconversion from woody biomass for biorefinery purposes.
本文描述了用于提高生物糖产量的行星式球磨机预处理和糖化工艺的优化。硬叶松（Pinus rigida）木屑废料被用作生物质原料，本研究中优化的工艺参数为缓冲介质、研磨时间、酶用量和孵育时间。使用醋酸盐缓冲液而非柠檬酸盐缓冲液时，葡萄糖产量有所提高。最初，通过对每个工艺变量进行测试，得出的最佳值为研磨100分钟、酶浓度为16 FPU/g生物质以及12小时的酶促水解。接下来，通常使用响应面法（RSM）研究这些实验条件之间的相互作用及其对葡萄糖生成的影响。在测试的几种条件下，硬叶松废料的葡萄糖产量超过了80%，这表明研磨可用于从木质生物质中获得高水平的葡萄糖生物转化，以用于生物精炼目的。
