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北江光唇鱼(鲤科,野鲮亚科)的完整线粒体基因组

The complete mitochondrial genome of Acrossocheilus beijiangensis (Cyprinidae, Barbinae).

作者信息

Lin Tingting, Li Qiang, Shu Hu, Yue Lei, Lin Jianfeng, Hou Liping

机构信息

a School of Life Science, Guangzhou University , Guangzhou , China.

出版信息

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4540-4542. doi: 10.3109/19401736.2015.1101553. Epub 2015 Nov 5.

Abstract

Acrossocheilus beijiangensis is a species of freshwater fish in the family Cyprinidae. In this study, the complete mitochondrial genome of A. beijiangensis has been determined using polymerase chain reaction technique. The genome is 16 600 bp in length, it includes 22 tRNA genes, 13 protein-coding genes, two rRNA genes, a D-loop control region, and another non-coding region called replication origin of Light strand (OL). The order of mitochondrial genes was found to be analogous to other vertebrates. The overall base composition of A. beijiangensis, mitochondrial DNA, was calculated and determined to consist of 30.6% A, 24.9% T, 28.1% C, and 16.3% G. Additionally it was found that most of the mitochondrial genes are encoded on the heavy strand except the NADH dehydrogenase subunit 6 (ND6) and eight tRNA genes. The availability of A. beijangesis mitogenome will provide basic data for the systematic categorization of Acrossocheilus sp., as well as the phylogenetic analysis of Cyprinid fishes.

摘要

北江光唇鱼是鲤科的一种淡水鱼。在本研究中,已使用聚合酶链反应技术测定了北江光唇鱼的完整线粒体基因组。该基因组长度为16600 bp,包括22个tRNA基因、13个蛋白质编码基因、2个rRNA基因、一个D环控制区以及另一个称为轻链复制起点(OL)的非编码区。发现线粒体基因的顺序与其他脊椎动物相似。计算并确定北江光唇鱼线粒体DNA的总体碱基组成为30.6%A、24.9%T、28.1%C和16.3%G。此外,还发现除了NADH脱氢酶亚基6(ND6)和8个tRNA基因外,大多数线粒体基因都由重链编码。北江光唇鱼线粒体基因组的可得性将为光唇鱼属的系统分类以及鲤科鱼类的系统发育分析提供基础数据。

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