Yeo F K S, Wang Y, Vozabova T, Huneau C, Leroy P, Chalhoub B, Qi X Q, Niks R E, Marcel T C
Laboratory of Plant Breeding, Wageningen University, Droevendaalsesteeg 1, 6708PB, 6700 AJ, Wageningen, The Netherlands.
Department of Plant Science and Environmental Ecology, Faculty of Resource Science and Technology, University Malaysia Sarawak, 94300, Kota Samarahan, Sarawak, Malaysia.
Theor Appl Genet. 2016 Feb;129(2):289-304. doi: 10.1007/s00122-015-2627-5. Epub 2015 Nov 5.
Rphq2, a minor gene for partial resistance to Puccinia hordei , was physically mapped in a 188 kbp introgression with suppressed recombination between haplotypes of rphq2 and Rphq2 barley cultivars.
Partial and non-host resistances to rust fungi in barley (Hordeum vulgare) may be based on pathogen-associated molecular pattern (PAMP)-triggered immunity. Understanding partial resistance may help to understand non-host resistance, and vice versa. We constructed two non-gridded BAC libraries from cultivar Vada and line SusPtrit. Vada is immune to non-adapted Puccinia rust fungi, and partially resistant to P. hordei. SusPtrit is susceptible to several non-adapted rust fungi, and has been used for mapping QTLs for non-host and partial resistance. The BAC libraries help to identify genes determining the natural variation for partial and non-host resistances of barley to rust fungi. A major-effect QTL, Rphq2, for partial resistance to P. hordei was mapped in a complete Vada and an incomplete SusPtrit contig. The physical distance between the markers flanking Rphq2 was 195 Kbp in Vada and at least 226 Kbp in SusPtrit. This marker interval was predicted to contain 12 genes in either accession, of which only five genes were in common. The haplotypes represented by Vada and SusPtrit were found in 57 and 43%, respectively, of a 194 barley accessions panel. The lack of homology between the two haplotypes probably explains the suppression of recombination in the Rphq2 area and limit further genetic resolution in fine mapping. The possible candidate genes for Rphq2 encode peroxidases, kinases and a member of seven-in-absentia protein family. This result suggests that Rphq2 does not belong to the NB-LRR gene family and does not resemble any of the partial resistance genes cloned previously.
Rphq2是对大麦柄锈菌具有部分抗性的一个微效基因，在一个188千碱基对的渗入片段中进行了物理定位，该片段中rphq2和Rphq2大麦品种单倍型之间的重组受到抑制。
大麦（Hordeum vulgare）对锈菌的部分抗性和非寄主抗性可能基于病原体相关分子模式（PAMP）触发的免疫反应。理解部分抗性可能有助于理解非寄主抗性，反之亦然。我们从品种Vada和品系SusPtrit构建了两个非网格化BAC文库。Vada对非适应性的柄锈菌免疫，对大麦柄锈菌具有部分抗性。SusPtrit对几种非适应性锈菌敏感，已被用于定位非寄主抗性和部分抗性的QTL。这些BAC文库有助于鉴定决定大麦对锈菌部分抗性和非寄主抗性自然变异的基因。一个对大麦柄锈菌具有部分抗性的主效QTL Rphq2被定位在一个完整的Vada重叠群和一个不完整的SusPtrit重叠群中。Rphq2两侧标记之间的物理距离在Vada中为195千碱基对，在SusPtrit中至少为226千碱基对。预计该标记区间在任何一个材料中都包含12个基因，其中只有5个基因是共同的。在一个由194个大麦材料组成的群体中，分别有57%和43%的材料具有Vada和SusPtrit所代表的单倍型。这两种单倍型之间缺乏同源性可能解释了Rphq2区域重组的抑制以及精细定位中进一步遗传分辨率的限制。Rphq2可能的候选基因编码过氧化物酶、激酶和七缺失蛋白家族的一个成员。这一结果表明，Rphq2不属于NB-LRR基因家族，也与之前克隆的任何部分抗性基因不同。
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