Yin Hongyu, Berdel Henrik O, Moore David, Davis Franklin, Liu Jun, Mozaffari Mahmood, Yu Jack C, Baban Babak
Department of Oral Biology, Georgia Regents University, Building: CL 2140, 1120 15th Street, Augusta, GA 30912 USA.
Plastic Surgery Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Peking, China.
Springerplus. 2015 Oct 6;4:578. doi: 10.1186/s40064-015-1373-0. eCollection 2015.
There is a worsening epidemic of obesity and diabetes in the world. Life style interventions including dietary changes and increase in exercise can improve glucose metabolism and health in general. However, standard exercise programs are strenuous, time-consuming, and thus have low long-term compliance issues. We tested the feasibility of using high frequency, low amplitude whole body vibration (WBV) therapy to improve glucose metabolism in young type 2 diabetic (T2DM) mice. We also aimed to investigate the postulated anti-inflammatory and cytoprotective properties of WBV. Male db/db and db/m mice were exposed to high frequency, low-amplitude WBV. Outcome parameters comprised of body weight, hemoglobin A1c (HbA1c) level, as well as interleukin (IL)-17 (a marker of helper T cells), forkhead box P3 (Foxp3; a marker of regulatory T cells), and gammaH2AX (an index of DNA injury) expression. Furthermore, a 24 h metabolic cage study was carried out immediately after the WBV protocol and fluid intake, urine excretion and urine osmolality were determined. WBV did not affect body weight but improved HbA1c levels in db/db mice. Vibrated db/db mice demonstrated less fluid intake and urine excretion but better urinary concentrating ability than their non-vibrated controls. Pro-inflammatory changes were significantly reduced, as indicated by reduced IL-17 but increased Foxp3 expression. WBV reduced gammaH2AX in db/db mice suggestive of cytoprotective effect. However, WBV was largely without significant effects on assessed parameters in db/m mice. Collectively, our findings suggest that daily, short duration WBV may improve glycemic control, polydipsia, polyuria, and urine osmolality in T2DM in association with reduced inflammation. Thus, WBV may be a viable adjunctive treatment strategy in T2DM.
全球肥胖和糖尿病的流行情况日益恶化。包括饮食改变和增加运动在内的生活方式干预总体上可改善葡萄糖代谢和健康状况。然而，标准的运动方案强度大、耗时，因此长期依从性较低。我们测试了使用高频、低振幅全身振动（WBV）疗法改善年轻2型糖尿病（T2DM）小鼠葡萄糖代谢的可行性。我们还旨在研究WBV假定的抗炎和细胞保护特性。将雄性db/db和db/m小鼠暴露于高频、低振幅WBV。结果参数包括体重、糖化血红蛋白（HbA1c）水平，以及白细胞介素（IL）-17（辅助性T细胞标志物）、叉头框P3（Foxp3；调节性T细胞标志物）和γH2AX（DNA损伤指标）的表达。此外，在WBV方案结束后立即进行24小时代谢笼研究，并测定液体摄入量、尿液排泄量和尿渗透压。WBV对db/db小鼠的体重没有影响，但改善了其HbA1c水平。与未振动的对照组相比，振动的db/db小鼠液体摄入量和尿液排泄量减少，但尿浓缩能力更好。促炎变化显著减少，表现为IL-17降低但Foxp3表达增加。WBV降低了db/db小鼠的γH2AX，提示具有细胞保护作用。然而，WBV对db/m小鼠的评估参数大多没有显著影响。总体而言，我们的研究结果表明，每日短时间的WBV可能与减轻炎症相关，从而改善T2DM患者的血糖控制、多饮、多尿和尿渗透压。因此，WBV可能是T2DM一种可行的辅助治疗策略。
