Khoshdel Abolfazl, Habibian Roya, Parvin Neda, Doosti Abbas, Famouri Fatemeh, Eshraghi Ali, Hafizi Massoud
Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Department of Infectious Diseases, Shahrekord University of Medical Sciences, Hajar Hospital, Parastar Street, Shahrekord, Iran.
Springerplus. 2015 Oct 19;4:627. doi: 10.1186/s40064-015-1268-0. eCollection 2015.
Clostridium difficile is recognized as a major cause of nosocomial acquired antibiotic-associated diarrhea and pseudomembranous colitis. It is a significant financial burden on modern healthcare resources. This study aimed to assess the molecular characterization of C. difficile strains isolated from children under 5 years old suffered from nosocomial diarrhea. One hundred diarrheic and 130 non-diarrheic fecal samples were collected from pediatrics less than 5 years old. Samples were cultured and C. difficile isolates were subjected to the PCR technique to study the distribution of ribotypes of C. difficile using P3 and P5 primers. Fifty-two out of 100 samples (52 %) were positive for C. difficile. The prevalence of bacterium in healthy children was 4.61 %. Total prevalence of C. difficile in diarrheic girls and boys were 48.9 and 54.7 %, respectively. Thirteen to twenty-four month age children had the highest prevalence of C. difficile. The most commonly detected ribotypes in the C. difficile isolates of Iranian pediatrics were RT027 (11.52 %), R1 (9.61 %) and R13 (7.68 %). The ribotypes of all of the six bacterial isolates of healthy children was not diagnosed. According to the presence of C. difficile and R27 ribotype, a continued genotype surveillance of this bacterium is necessary to monitor changes in the prevalence of certain strains and to identify the emergence of new strains that could affect future vaccine strategies.
艰难梭菌被认为是医院获得性抗生素相关性腹泻和假膜性结肠炎的主要病因。它给现代医疗资源带来了巨大的经济负担。本研究旨在评估从5岁以下患医院感染性腹泻的儿童中分离出的艰难梭菌菌株的分子特征。从5岁以下的儿科患者中收集了100份腹泻粪便样本和130份非腹泻粪便样本。对样本进行培养，并使用P3和P5引物对艰难梭菌分离株进行PCR技术检测，以研究艰难梭菌核糖体分型的分布。100份样本中有52份（52%）艰难梭菌检测呈阳性。健康儿童中该细菌的患病率为4.61%。腹泻女童和男童中艰难梭菌的总患病率分别为48.9%和54.7%。13至24个月大的儿童艰难梭菌患病率最高。伊朗儿科患者艰难梭菌分离株中最常检测到的核糖体分型为RT027（11.52%）、R1（9.61%）和R13（7.68%）。未诊断出所有6份健康儿童细菌分离株的核糖体分型。根据艰难梭菌和R27核糖体分型的存在情况，有必要对该细菌进行持续的基因型监测，以监测某些菌株患病率的变化，并识别可能影响未来疫苗策略的新菌株的出现。
