Food and Feed Safety Research Unit, SPARC, Agricultural Research Service, U.S. Department of Agriculture, College Station, Texas 77845, USA.
Foodborne Pathog Dis. 2011 Dec;8(12):1321-3. doi: 10.1089/fpd.2011.0936. Epub 2011 Aug 30.
The incidence and severity of disease associated with toxigenic Clostridium difficile have increased in hospitals in North America from the emergence of newer, more virulent strains. Toxigenic C. difficile has been isolated from food animals and retail meat with potential implications of transfer to human beings. The objective of the present study was to determine the prevalence of toxigenic C. difficile in chickens and retail poultry meat in Texas. Seven C. difficile isolates were detected in fecal samples of 300 (2.3%) broiler chickens. Three cultivation procedures were evaluated for isolation of C. difficile from poultry meat and detected 1/32 (3.1%), 2/32 (6.2%), and 4/32 (12.5%) for the three procedures, respectively. Chicken and poultry meat isolates were characterized as toxinotype V and pulsed-field gel electrophoresis gel type-NAP7 or NAP7-variant. Susceptibilities to 11 antimicrobial agents in the current study suggested somewhat reduced resistance than reported for other meat or animal toxinotype V isolates.
产毒艰难梭菌相关疾病的发病率和严重程度，自北美新型、高毒力菌株出现以来，在医院内有所增加。产毒艰难梭菌已从食用动物和零售肉类中分离出来，可能会转移到人类身上。本研究的目的是确定德克萨斯州鸡和零售禽肉中产毒艰难梭菌的流行情况。在 300 只（2.3%）肉鸡的粪便样本中检测到 7 株艰难梭菌分离株。三种培养程序用于从禽肉中分离艰难梭菌，分别检测到 1/32（3.1%）、2/32（6.2%）和 4/32（12.5%）。鸡和禽肉分离株的毒素型为 V 型，脉冲场凝胶电泳凝胶型为 NAP7 或 NAP7-变体。本研究中对 11 种抗菌药物的敏感性表明，与其他肉类或动物毒素 V 型分离株的耐药性相比，耐药性略有降低。
