Wang Xin-Hong, Li Li-Jun, Sun Gui-Xin, Wu Zuo-Pei, Li Ji-Feng, Gu Yu-Dong
aDepartment of Physiology & Pathophysiology, School of Basic Medical Sciences bDepartment of Hand Surgery, Huashan Hospital, Fudan University cDepartment of Spine Surgery dDepartment of Traumatology, Shanghai East Hospital, Tongji University, Shanghai, People's Republic of China.
Neuroreport. 2016 Jan 6;27(1):12-7. doi: 10.1097/WNR.0000000000000485.
The transfer of a contralateral healthy seventh cervical spinal nerve root (cC7) to the recipient nerve in the injured side is considered a promising procedure for restoration of the physiological functions of an injured hand after brachial plexus root avulsion injury (BPAI). Growing evidence shows that transhemispheric cortical reorganization plays an important role in the functional recovery of the injured arm after cC7 nerve transfer surgery. However, the molecular mechanism underlying the transhemispheric cortical reorganization after cC7 transfer remains elusive. In the present study, we investigated the expression of miR-132, miR-134, and miR-485 in the rat primary motor cortex after cC7 transfer following BPAI by quantitative PCR. The results demonstrated the dynamic alteration in the expression of miR-132, miR-134, and miR-485 in the primary motor cortex of rats after cC7 transfer following BPAI. It indicates that microRNAs are involved in the dynamic transhemispheric functional reorganization after cC7 root transfer following BPAI. Together, this study is the first to provide evidence for the involvement of microRNAs during dynamic transhemispheric functional reorganization after cC7 transfer following BPAI. The results are useful for understanding the mechanism underlying transhemispheric functional reorganization after contralateral seventh cervical spinal nerve root transfer following BPAI.
将对侧健康的第七颈脊髓神经根（cC7）转移至损伤侧的受区神经，被认为是一种有望恢复臂丛神经根性撕脱伤（BPAI）后受伤手部生理功能的手术方法。越来越多的证据表明，跨半球皮质重组在cC7神经转移手术后受伤手臂的功能恢复中起重要作用。然而，cC7转移后跨半球皮质重组的分子机制仍不清楚。在本研究中，我们通过定量PCR检测了BPAI后cC7转移大鼠初级运动皮质中miR-132、miR-134和miR-485的表达。结果显示，BPAI后cC7转移大鼠初级运动皮质中miR-132、miR-134和miR-485的表达发生动态变化。这表明微小RNA参与了BPAI后cC7神经根转移后的动态跨半球功能重组。总之，本研究首次为微小RNA参与BPAI后cC7转移后的动态跨半球功能重组提供了证据。这些结果有助于理解BPAI后对侧第七颈脊髓神经根转移后跨半球功能重组的机制。
