[用于显微凝集的钩端螺旋体菌株精简组评估]
[Evaluation of a reduced panel of leptospira strains for microagglutination].
作者信息
Jacob Paulina, Schmeling María F, Chiani Yosena T, Landolt Noelia Y, Scialfa Exequiel, Fusco Silvia, Vanasco Bibiana
机构信息
Laboratorio de Leptospirosis, Instituto Nacional de Enfermedades Respiratorias Dr. Emilio Coni, Santa Fe, Argentina.
Departamento de Zoonosis Rurales, Ministerio de Salud de la Provincia de Buenos Aires, Buenos Aires, Argentina.
出版信息
Salud Publica Mex. 2015 Sep-Oct;57(5):419-25.
OBJECTIVE
To evaluate if the use of the 19 Leptospira strains panel suggested by the International Leptospirosis Society of World Health Organization for microagglutination allows confirmation of more cases that the 12 strains panel used in Argentina.
MATERIALS AND METHODS
Cross-sectional observational study. We studied 441 serum samples corresponding to Argentinean patients with suspected leptospirosis derived during from July to December, 2009 and from January to October, 2013.
RESULTS
The same number of positive samples was obtained using the MAT with the 19 or 12 strains. In six cases a serovar of the expanded collection was presumably infecting, but always coagglutinated with strains of the reduced panel.
CONCLUSION
In Argentina, the diagnosis of leptospirosis by MAT could be made using the reduced 12 strains panel, obtaining the same result in case detection as using the 19 strains panel. Additional information provided by the use of all strains could be the presumably infecting serogroup.
目的
评估世界卫生组织国际钩端螺旋体病协会建议用于显微凝集试验的19种钩端螺旋体菌株组合是否比阿根廷使用的12种菌株组合能确诊更多病例。
材料与方法
横断面观察性研究。我们研究了441份血清样本，这些样本来自2009年7月至12月以及2013年1月至10月期间疑似钩端螺旋体病的阿根廷患者。
结果
使用19种或12种菌株进行显微凝集试验获得的阳性样本数量相同。在6例病例中，推测感染的是扩展菌株组合中的一种血清型，但总是与简化菌株组合中的菌株发生协同凝集。
结论
在阿根廷，通过显微凝集试验诊断钩端螺旋体病可使用简化的12种菌株组合，在病例检测方面与使用19种菌株组合获得相同结果。使用所有菌株提供的额外信息可能是推测感染的血清群。
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