Hasegawa Akiyuki, Haraguchi Yuji, Oikaze Hirotoshi, Kabetani Yasuhiro, Sakaguchi Katsuhisa, Shimizu Tatsuya
Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women's Medical University, Tokyo, Japan.
Panasonic Corporation, Osaka, Japan.
J Biomed Mater Res B Appl Biomater. 2017 Apr;105(3):481-488. doi: 10.1002/jbm.b.33566. Epub 2015 Nov 6.
Optical coherence tomography (OCT) is a valuable tool in the cross-sectional observation/analysis of three-dimensional (3-D) biological tissues, and that histological observation is important clinically. However, the resolution of the technology is approximately 10-20 μm. In this study, optical coherence microscopy (OCM), a tomographic system combining OCT technology with a microscopic technique, was constructed for observing cells individually with a resolution at the submicrometer level. Cells and 3-D tissues fabricated by cell sheet technology were observed by OCM. Importantly, the cell nuclei and cytoplasm could be clearly distinguished, and the time-dependent dynamics of cell-sheet tissues could be observed in detail. Additionally, the 3-D migration of cells in the bioengineered tissue was also detected using OCM and metal-labeled cells. Bovine aortic endothelial cells, but not NIH3T3 murine embryonic skin fibroblasts, actively migrated within the 3-D tissues. This study showed that the OCM system would be a valuable tool in the fields of cell biology, tissue engineering, and regenerative medicine. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 481-488, 2017.
光学相干断层扫描（OCT）是用于三维（3-D）生物组织横断面观察/分析的一种重要工具，而组织学观察在临床上也很重要。然而，该技术的分辨率约为10 - 20μm。在本研究中，构建了一种光学相干显微镜（OCM），这是一种将OCT技术与显微技术相结合的断层扫描系统，用于以亚微米级分辨率单独观察细胞。通过OCM观察了利用细胞片技术制备的细胞和3-D组织。重要的是，能够清晰区分细胞核和细胞质，并且可以详细观察细胞片组织随时间的动态变化。此外，还使用OCM和金属标记细胞检测了生物工程组织中细胞的3-D迁移。牛主动脉内皮细胞而非NIH3T3小鼠胚胎皮肤成纤维细胞在3-D组织内积极迁移。本研究表明，OCM系统将成为细胞生物学、组织工程和再生医学领域的一种重要工具。© 2015威利期刊公司。《生物医学材料研究杂志》B部分：应用生物材料，105B：481 - 488，2017。
