离散人前列腺细胞群体的分离与分析。
Isolation and analysis of discreet human prostate cellular populations.
作者信息
Strand Douglas W, Aaron LaTayia, Henry Gervaise, Franco Omar E, Hayward Simon W
机构信息
Department of Urology, UT Southwestern University Medical Center, Dallas, TX, USA.
Department of Cancer Biology, Meharry Medical College, Nashville, TN, USA.
出版信息
Differentiation. 2016 Apr-Jun;91(4-5):139-51. doi: 10.1016/j.diff.2015.10.013. Epub 2015 Nov 3.
Abstract
The use of lineage tracing in transgenic mouse models has revealed an abundance of subcellular phenotypes responsible for maintaining prostate homeostasis. The ability to use fresh human tissues to examine the hypotheses generated by these mouse experiments has been greatly enhanced by technical advances in tissue processing, flow cytometry and cell culture. We describe in detail the optimization of protocols for each of these areas to facilitate research on solving human prostate diseases through the analysis of human tissue.
摘要
在转基因小鼠模型中使用谱系追踪技术已经揭示了大量负责维持前列腺内环境稳定的亚细胞表型。组织处理、流式细胞术和细胞培养等技术的进步极大地提高了利用新鲜人体组织检验这些小鼠实验所产生假设的能力。我们详细描述了这些领域中每个领域方案的优化，以促进通过对人体组织的分析来解决人类前列腺疾病的研究。
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