Huang Liangliang, Li Meicai, Wang Delin, He Jiang, Wu Wenqiang, Zeng Qiangfeng, Li Jianjun, Xiao Maolin, Hu Jie, He Yunfeng, Li Ying, Mai Li, Liu Wujiang
Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, Chongqing, 400016, People's Republic of China.
Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, Chongqing, 400016, People's Republic of China.
Hum Pathol. 2016 Jan;47(1):109-14. doi: 10.1016/j.humpath.2015.08.021. Epub 2015 Sep 28.
Ras and a-factor-converting enzyme 1 (Rce1) have been reported to play a key role in the proteolysis processing of Ras proteins. The present study investigated the prognostic significance of Rce1 in patients with prostate cancer (PCa). The expressions of the mRNA and protein of Rce1 were analyzed in 12 pairs of PCa and benign prostatic hyperplasia (BPH) by quantitative real-time polymerase chain reaction and Western blotting, respectively. Immunohistochemistry was used to examine expression of Rce1 protein in 74 PCa tissues and 30 BPH tissues. The association between Rce1 expression and the specific clinicopathologic features was evaluated by χ(2) tests. Kaplan-Meier and Cox proportional hazards regression models were used to analyze the data. We found that expression of Rce1 mRNA and protein was markedly higher in PCa tissues than in paired BPH tissues. Expression of Rce1 in PCa was strongly associated with clinicopathologic features. It was detected in 69 (93.24%) of 74 PCa tissues by immunohistochemistry, and it was found to be associated with Gleason score (P = .013), T class (P = .015), and distant metastasis (P = .044). Patients with PCa having higher Rce1 expression had substantially shorter survival times than patients with lower Rce1 expression. Univariate and multivariate analysis revealed that Rce1 was an independent prognostic factor. In conclusion, our study suggests that expression of Rce1 can serve as an independent biomarker for the prognosis of PCa patients.
据报道，Ras和α-因子转化酶1（Rce1）在Ras蛋白的蛋白水解加工过程中起关键作用。本研究调查了Rce1在前列腺癌（PCa）患者中的预后意义。分别通过定量实时聚合酶链反应和蛋白质印迹法分析了12对PCa和良性前列腺增生（BPH）中Rce1的mRNA和蛋白表达。采用免疫组织化学法检测74例PCa组织和30例BPH组织中Rce1蛋白的表达。通过χ²检验评估Rce1表达与特定临床病理特征之间的关联。使用Kaplan-Meier法和Cox比例风险回归模型分析数据。我们发现，PCa组织中Rce1 mRNA和蛋白的表达明显高于配对的BPH组织。PCa中Rce1的表达与临床病理特征密切相关。免疫组织化学法在74例PCa组织中的69例（93.24%）中检测到Rce1，发现其与Gleason评分（P = .013）、T分期（P = .015）和远处转移（P = .044）相关。Rce1表达较高的PCa患者的生存时间明显短于Rce1表达较低的患者。单因素和多因素分析显示，Rce1是一个独立的预后因素。总之，我们的研究表明，Rce1的表达可作为PCa患者预后的独立生物标志物。
