Ding Yipeng, Yang Hua, He Haiwu, Shi Huifang, He Ping, Yan Shouchun, Jin Tianbo
Emergency Department of Hainan Provincial People's Hospital , Haikou, 570102, China.
School of Life Sciences, Northwest University, Xi'an 710069, China.
Iran J Allergy Asthma Immunol. 2015 Jun;14(3):292-7.
The purpose of this study was to determine the clinical significance of changes in the plasma adiponectin concentration in patients with bronchial asthma and to test the association between the single nucleotide polymorphisms (SNPs) rs2241766 and rs1501299 in the ADIPOQ gene and bronchial asthma in the Chinese Li population. We selected 120 cases and 120 controls, and plasma adiponectin, interleukin (IL)-6, and tumor necrosis factor-alpha (TNF-α) levels were measured by enzyme-linked immunosorbent assay (ELISA). In addition, we genotyped two tag single nucleotide polymorphisms (tSNPs) and evaluated their association with bronchial asthma using the χ2 test and genetic model analysis. Compared to controls, patients with acute exacerbation of bronchial asthma showed significantly lower adiponectin and significantly higher IL-6 and TNF-α levels (p<0.01). A positive association was found between the rs1501299 SNP and acute exacerbation (OR=1.62; 95% CI=1.08-2.43; p=0.019). The inverse correlation between the plasma adiponectin concentration and asthma exacerbation indicates that adiponectin may play a protective role in the pathogenesis of asthma. Meanwhile, our findings suggest that ADIPOQ polymorphisms influence the risk of developing bronchial asthma in Chinese Li population.
本研究的目的是确定支气管哮喘患者血浆脂联素浓度变化的临床意义,并检测脂联素基因(ADIPOQ)中的单核苷酸多态性(SNP)rs2241766和rs1501299与中国黎族人群支气管哮喘之间的关联。我们选取了120例病例和120例对照,采用酶联免疫吸附测定(ELISA)法检测血浆脂联素、白细胞介素(IL)-6和肿瘤坏死因子-α(TNF-α)水平。此外,我们对两个标签单核苷酸多态性(tSNP)进行基因分型,并使用χ2检验和遗传模型分析评估它们与支气管哮喘的关联。与对照组相比,支气管哮喘急性加重患者的脂联素水平显著降低,IL-6和TNF-α水平显著升高(p<0.01)。发现rs1501299 SNP与急性加重之间存在正相关(OR=1.62;95%CI=1.08-2.43;p=0.019)。血浆脂联素浓度与哮喘加重之间的负相关表明脂联素可能在哮喘发病机制中起保护作用。同时,我们的研究结果表明,ADIPOQ基因多态性影响中国黎族人群患支气管哮喘的风险。