Türkoğlu Oya, Eren Gülnihal, Emingil Gülnur, Azarsız Elif, Kutukculer Necil, Atilla Gül
Department of Periodontology, School of Dentistry, Ege University, Izmir, Turkey.
Department of Pediatrics, School of Medicine, Ege University, Izmir, Turkey.
Arch Oral Biol. 2016 Jan;61:98-105. doi: 10.1016/j.archoralbio.2015.10.018. Epub 2015 Oct 27.
LL-37 contributes to maintaining the balance between health and disease. Smoking is a risk factor for periodontitis that impairs neutrophil functions. The aim of the present study was to comparatively evaluate gingival crevicular fluid (GCF) LL-37 levels in smoker and non-smoker chronic periodontitis (CP) patients and controls, as well as the effect of non-surgical periodontal treatment on GCF LL-37 levels.
Thirty-one CP patients (16 smokers, 15 non-smokers) and thirty-one controls (16 smokers, 15 non-smokers) were included in the study. CP patients received non-surgical treatment. GCF LL-37 levels and periodontal parameters were assessed at baseline, 1 and 3 months after completion of non-surgical periodontal treatment. GCF LL-37 levels were analyzed by ELISA.
No significant difference was observed in GCF LL-37 levels between smoker and non-smoker controls (p>0.05). Smoker CP group had significantly lower GCF LL-37 level than non-smoker CP group at baseline (p<0.05). GCF LL-37 levels significantly decreased in non-smoker CP group at first week, 1 and 3 months after completion of non-surgical periodontal treatment (p<0.05) although no significant decrease in GCF LL-37 levels was observed in smoker CP group (p>0.05). Periodontal parameters were correlated with GCF LL-37 levels in non-smoker CP group (p<0.05), but not in smoker CP group (p>0.05).
GCF LL-37 levels do not seem to be affected from smoking in periodontal health. However, smoking might have a suppressive effect on GCF LL-37 levels in CP. Non-surgical treatment is effective in decreasing GCF LL-37 levels in non-smoker CP patients but not in smokers with CP.
LL-37有助于维持健康与疾病之间的平衡。吸烟是牙周炎的一个风险因素，会损害中性粒细胞功能。本研究的目的是比较评估吸烟者和非吸烟者慢性牙周炎（CP）患者及对照者龈沟液（GCF）中LL-37水平，以及非手术牙周治疗对GCF中LL-37水平的影响。
本研究纳入31例CP患者（16例吸烟者，15例非吸烟者）和31例对照者（16例吸烟者，15例非吸烟者）。CP患者接受非手术治疗。在基线、非手术牙周治疗完成后1个月和3个月时评估GCF中LL-37水平和牙周参数。采用酶联免疫吸附测定（ELISA）法分析GCF中LL-37水平。
吸烟者和非吸烟者对照者之间GCF中LL-37水平未观察到显著差异（p>0.05）。在基线时，吸烟者CP组的GCF中LL-37水平显著低于非吸烟者CP组（p<0.05）。非吸烟者CP组在非手术牙周治疗完成后第1周、1个月和3个月时GCF中LL-37水平显著降低（p<0.05），而吸烟者CP组未观察到GCF中LL-37水平有显著降低（p>0.05）。在非吸烟者CP组中，牙周参数与GCF中LL-37水平相关（p<0.05），但在吸烟者CP组中不相关（p>0.05）。
在牙周健康状态下，GCF中LL-37水平似乎不受吸烟影响。然而，吸烟可能对CP患者GCF中LL-37水平有抑制作用。非手术治疗对降低非吸烟者CP患者GCF中LL-37水平有效，但对吸烟者CP患者无效。
