Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; University of Chinese Academy of Sciences, Beijing 100039, China.
Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Bioresour Technol. 2016 Jan;200:598-605. doi: 10.1016/j.biortech.2015.10.059. Epub 2015 Oct 23.
Lipids and carbohydrates are main energy storage compounds (ESC) of microalgae under stressed conditions and they are potential feedstock for biofuel production. Yet, the sustainable and commercially successful production of ESC in microalgae needs to consider nitrogen utilization efficiency. Here the impact of different initial cell densities (ICDs) on ESC accumulation in Isochrysis zhangjiangensis under two nitrogen supply modes (an initially equal concentration of nitrogen per-cell in the medium (N1) and an equal total concentration of nitrogen in the culture system (N2)) were investigated. The results demonstrated that the highest ESC yield (1.36gL(-1)) at N1, which included a maximal nitrogen supply in the cultivation system, and the highest ESC content (66.5%) and ESC productivity per mass of nitrogen (3.28gg(-1) (N) day(-1)) at N2, were all obtained under a high ICD of 8.0×10(6)cellsmL(-1). Therefore I. zhangjiangensis qualifies for ESC-enriched biomass production with economized nitrogen utilization.
脂质和碳水化合物是微藻在胁迫条件下的主要能量储存化合物（ESC），它们是生物燃料生产的潜在原料。然而，要实现 ESC 在微藻中的可持续和商业上的成功生产，就需要考虑氮的利用效率。在这里，研究了在两种氮供应模式（培养基中每细胞初始氮浓度相等（N1）和培养系统中总氮浓度相等（N2））下，不同初始细胞密度（ICD）对中肋骨条藻 ESC 积累的影响。结果表明，在 ICD 为 8.0×10^6cellsmL^(-1)时，N1 条件下获得了最高的 ESC 产率（1.36gL^(-1)），其中包括培养系统中最高的氮供应，而在 N2 条件下获得了最高的 ESC 含量（66.5%）和每单位氮质量的 ESC 生产力（3.28gg^(-1)（N）day^(-1)）。因此，中肋骨条藻适合用于生产富含 ESC 的生物量，并节约氮的利用。
