Ota Yusuke, Matsumoto Takehisa, Sugano Mitsutoshi, Honda Takayuki
Rinsho Byori. 2015 Jun;63(6):683-7.
In this study, the performance of MALDI-TOF MS was evaluated for the identification of clinical thymidine-dependent small-colony variants (TD-SCVs) of Staphylococcus aureus. We performed identification of a total of 15 S. aureus TD-SCVs by using biochemical tests, 16S rRNA gene sequencing and MALDI-TOF MS analysis. Although the biochemical method using MicroScan panels could not identify all isolates due to insufficient growth in the control well. MALDI Biotyper (Bruker Daltonics) could correctly identify all of them. Two sample preparation methods, the direct transfer-formic acid method and ethanol-formic acid method, for measurement by MALDI Biotyper made no difference in results. MALDI-TOF MS is useful identification of S. aureus TD-SCVs.
在本研究中，对基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）用于鉴定金黄色葡萄球菌临床胸腺嘧啶依赖性小菌落变体（TD-SCVs）的性能进行了评估。我们通过生化试验、16S rRNA基因测序和MALDI-TOF MS分析对总共15株金黄色葡萄球菌TD-SCVs进行了鉴定。尽管使用MicroScan板的生化方法由于对照孔中生长不足而无法鉴定所有分离株，但MALDI Biotyper（布鲁克道尔顿公司）能够正确鉴定所有分离株。用于MALDI Biotyper测量的两种样品制备方法，即直接转移-甲酸法和乙醇-甲酸法，在结果上没有差异。MALDI-TOF MS对于金黄色葡萄球菌TD-SCVs的鉴定很有用。
